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Product Info Summary
| SKU: | A00740 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-CARMA1 CARD11 Antibody
View all CARD11/CARMA1 Antibodies
SKU/Catalog Number
A00740
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CARMA1 CARD11 Antibody (Catalog # A00740). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
CARMA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-CARMA1 CARD11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00740)
Host
Rabbit
Contents
CARMA1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CARMA1 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human CARMA1. The immunogen is located within the last 50 amino acids of CARMA1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
ROCK1 antibody is predicted to not cross-react with other ROCK protein family members.
Reactive Species
A00740 is reactive to CARD11 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
133284 MW
Background of CARD11/CARMA1
CARMA proteins belong to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARMA1, CARMA2, and CARMA3 share high degrees of sequence and functional homology, but their tissue-specific distribution suggests that they serve distinct biological functions in different cell types. Both CARMA1 and CARMA3 associate with NEMO, the regulatory subunit of the IκK complex, thereby regulating activation of the NF-κB transcription factor. Also, gene inactivation studies showed a complete block in T and B cell immunity as well as an impaired response to LPS, indicating that CARMA1 is a critical regulator in both the adaptive and innate immune systems.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00740 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CARMA1 antibody can be used for detection of CARMA1 by Western blot at 0.5 to 2 μg/mL. A band at approximately 125 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CARMA1 expression in mouse thymus cell lysate with CARMA1 antibody at 0.5 (lane A)
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Immunohistochemistry of CARMA1 in mouse thymus with CARMA1 antibody at 10 μg/mL.
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Immunofluorescence of CARMA1 in Mouse Spleen cells with CARMA1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For CARD11 (Source: Uniprot.org, NCBI)
Gene Name
CARD11
Full Name
Caspase recruitment domain-containing protein 11
Weight
133284 MW
Alternative Names
PPBL, BENTA, BIMP3, IMD11, CARMA1, Caspase recruitment domain-containing protein 11, CARD-containing MAGUK protein 1, Carma 1 CARD11 BENTA, BIMP3, CARMA1, IMD11, IMD11A, PPBL caspase recruitment domain family member 11 caspase recruitment domain-containing protein 11|CARD-containing MAGUK protein 1|bcl10-interacting maguk protein 3|carma 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CARD11, check out the CARD11 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CARD11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CARMA1 CARD11 Antibody (A00740)
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