NLRC4 Antibodies

NLR family CARD domain-containing protein 4 (NLRC4)

Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and reacts by building an inflammasome complex that boosts caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed:15107016). The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria (By similarity).

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Gene Information

Human
Gene Name:	NLRC4
Uniprot:	Q9NPP4
Entrez:	58484

Family

Belongs to:
No superfamily

Alternative Names

CARD12leucine rich repeat and CARD domaincontaining 4; caspase recruitment domain family, member 12; Caspase recruitment domain-containing protein 12; Clan protein; CLAN1ipaf; CLANCARD, LRR, and NACHT-containing protein; CLR2.1; Ice protease-activating factor; Ipaf; NLR family, CARD domain containing 4

Molecular Weight

Mass (kDA):
116.159 kDA

Genomic Context

Human
Location:	2p22.3
Sequence:	2; NC_000002.12 (32224449..32265743, complement)

Tissue Specificity

Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain.

Subcellular Localizations

Cytoplasm. Cytoplasm, cytosol. Cytoplasmic filaments.

Diseases

• Inflammation
• Salmonella Infections
• Legionnaires' Disease
• Bacterial Infections
• Innate Immune Response
• Infective Disorder
• Malignant Neoplasms
• Pneumonia
• Neoplasms
• Pseudomonas Infections
• Shigella Infections
• Permissiveness, Biological Function
• Disease Caused By Shigella Flexneri

• Inflammatory Response
• Cell Invasion
• Gram-negative Bacterial Infections
• Autoimmune Diseases
• Peritonitis
• Colitis
• Cell Transformation, Neoplastic

Interacting Proteins

• CASP1

Pathways

• Secretion
• Cell Death
• Pyroptosis
• Immune Response
• Pathogenesis
• Innate Immune Response
• Virulence
• Inflammatory Response
• Cytokine Production
• Rna Interference
• Autophagy
• Transport
• Programmed Cell Death

• Phagosome Maturation
• Epithelial Cell Proliferation
• Cell Proliferation
• Dna Repair
• Tissue Homeostasis
• Gene Silencing
• Phagosome Acidification

PTMs

• Cleavage
• Phosphorylation

• Nitrosylation
• Ubiquitination

Research Areas

• Apoptosis
• Immunology
• Innate Immunity

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NLRC4

Best Uses of the NLRC4 Marker

The NLRC4 Marker is a peptide that targets cancer and is a boster product. This peptide could be used to kill and detect cancer cells as the primary antibody. Boster Bio has been providing high affinity primary antibodies as well as chemicals for cancer research for more than 10 years. Learn more about the NLRC4 Marker and its applications.

Boster offers high-affinity primary antibodies

The BACE inhibitor NRLC4 has recently been found to block the activity of CD8 T cells. which is a molecule found on the T cell's surface and is a critical component of the T cells subset. T cells comprise about 20-30% of the lymphocytes in the peripheral blood. CD8 antigen is present on 80% of thymocytes, natural killer cells, and bone marrow cells. Boster Bio provides high-affinity primary antibodies against this marker that can aid in your research.

NLRC4 Marker is a cancer-specific peptide

The NLRC4 Marker is a distinct molecule that's presence and activity can identify or target a particular type of cancer. Its unique cell-penetrating capabilities make it a suitable delivery system for chemotherapy. This research provides important new insights into the field of cancer immunotherapy. The peptide may also be beneficial in the development of more effective chemotherapy drugs.

The NLRC4 Inflammasome comprises of three parts including NLRC4, a receptor as well as the sensor ASC and the adaptor. ASC is recruited into the inflammasome in the aftermath of infection by S. typhimurium. Casparase-1 also gets recruited by this infection. Inflammasomes can be seen in the cytoplasm, and they are dissolvable. ASC is a soluble constituent that forms a massive paranuclear-speck with a diameter of around 1mm.

NLRC4 Acetylation has been identified in HEK293T cells that were transfected with Flag-tagged NLRC4 proteins. The mass spectrometer was utilized to examine the immunoprecipitates. The sequences of NLRC4 in seven species were aligned and the acetylated residues (K71 K272, K674 and K763) were highlighted in red.

Applications

The role of the NLRC4 marker in bacterial enteric inflammation has been extensively studied. It is vital for the removal and function bacteria. NLRC4 is expressed at basal levels in epithelial and immune cells. The expression of this marker is controlled by phosphorylation, ligand binding, and is regulated by the process of phosphorylation. This article will discuss some of the applications of this marker. We conclude that NLRC4 can be used to study the immune system's innate components.

NLRC4 is part of the AAA+ ATPase family. It has structural features common to other NLR proteins. It has a central NTPase domain, as well as two helical domains NLRR or LRR. The NLRC4 marker's C-terminal amino acid 440 is composed of 15 repeating structural units. Each unit is comprised of a beta strand that is leucine-rich and joined to an alpha helix that is short and short.

Inflammasomes are one of the major causes of inflammation in human diseases. Inflammasomes possess a variety of distinct structural and functional properties and are involved in a wide range of inflammation-related diseases. NLRC4 is a part of the inflammasome. It is responsible for the cleavage of a variety substrates, including the cytosolic flagellin. It triggers a variety of autoinflammatory processes that cause cell death. Gain-of function mutations are found near the ADP binding site of the NLRC4 gene, and are associated with skin rash, enterolitis, and chronically high levels of IL-18.

There are a variety of clinical applications for the NLRC4 marker. The protein is linked to the inflammasome, and co-localizes well with NLRP3 genes. NLRC4 is also co-localized with NLRP3 in vivo. However, the super-resolution microscope used in these experiments does not provide enough resolution to discern specific protein-protein interactions. IL-18 and NLRC4 also interact with each other.

This research could have implications for a range of diseases like type 2 diabetes and systemic Lupus. These findings could also prove beneficial to other areas of medicine. These studies are just the beginning. It is vital that we continue studying this complicated marker to better understand how the disease is developing. A person could develop a treatment for this disease when NLRC4 isn't functioning correctly.

The variants of NLRC4's gene were selected from the most current literature in accordance with their minor-allele frequency. The Asian population must have the minor allele frequency higher than 0.05. Furthermore, the selected SNPs were not located in the same region of linkage. The markers of NLRC4 were genotyped using MassARRAY and PCR primers designed by Agena Bioscience. Table 1 shows the sequences of the primers.