Product Info Summary
SKU: | A00495-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Monkey |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-CARD4/NOD1 Antibody Picoband™
SKU/Catalog Number
A00495-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CARD4/NOD1 Antibody Picoband™ catalog # A00495-1. Tested in WB applications. This antibody reacts with Monkey.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CARD4/NOD1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00495-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CARD4/NOD1, which shares 88.2% and 93.8% amino acid (aa) sequence identity with mouse and rat CARD4/NOD1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00495-1 is reactive to NOD1 in Monkey
Applications
A00495-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
108 kDa
Calculated molecular weight
29159 MW
Background of NOD1
Nucleotide-binding oligomerization domain-containing protein 1, also known as CARD4, is a protein receptor that in humans is encoded by the NOD1 gene. NOD1 is a member of NOD-like receptor protein family and is a close relative of NOD2. NOD1 is mapped to 7p14.3. It recognizes bacterial molecules and stimulates an immune reaction. NOD1 protein contains a caspase recruitment domain (CARD). This gene is an intracellular pattern recognition receptor, which is similar in structure to resistant proteins of plants, and mediates innate and acquired immunity by recognizing bacterial molecules containing D-glutamyl-meso-diaminopimelic acid (iE-DAP) moiety. What wore, it has been shown that NOD1 can sense cytosolic microbial products by monitoring the activation state of small Rho GTPases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Monkey
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CARD4/NOD1 using anti-CARD4/NOD1 antibody (A00495-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: monkey heart tissue lysates,
Lane 2: monkey skeletal muscle tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CARD4/NOD1 antigen affinity purified polyclonal antibody (Catalog # A00495-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CARD4/NOD1 at approximately 108KD. The expected band size for CARD4/NOD1 is at 108KD.
Protein Target Info & Infographic
Gene/Protein Information For NOD1 (Source: Uniprot.org, NCBI)
Gene Name
NOD1
Full Name
Nucleotide-binding oligomerization domain-containing protein 1
Weight
29159 MW
Alternative Names
CARD4; CARD4NLR family, CARD domain containing 1; caspase recruitment domain family, member 4; Caspase recruitment domain-containing protein 4; CLR7.1; NLRC1; NOD1; nucleotide-binding oligomerization domain containing 1; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domaincontaining 1; nucleotide-binding oligomerization domain-containing protein 1 NOD1 CARD4, CLR7.1, NLRC1 nucleotide binding oligomerization domain containing 1 nucleotide-binding oligomerization domain-containing protein 1|NLR family, CARD domain containing 1|caspase recruitment domain family, member 4|caspase recruitment domain-containing protein 4|nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NOD1, check out the NOD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NOD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CARD4/NOD1 Antibody Picoband™ (A00495-1)
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