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Product Info Summary
| SKU: | PB9294 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-CARD4/NOD1 Antibody Picoband®
SKU/Catalog Number
PB9294
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CARD4/NOD1 Antibody Picoband® catalog # PB9294. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CARD4/NOD1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9294)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CARD4 recombinant protein (Position: M1-M160). Human CARD4 shares 82% amino acid (aa) sequence identity with mouse CARD4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9294 is reactive to NOD1 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
107 kDa
Calculated molecular weight
107691 MW
Background of NOD1
Nucleotide-binding oligomerization domain-containing protein 1, also known as CARD4, is a protein receptor that in humans is encoded by the NOD1 gene. NOD1 is a member of NOD-like receptor protein family and is a close relative of NOD2. NOD1 is mapped to 7p14.3. It recognizes bacterial molecules and stimulates an immune reaction. NOD1 protein contains a caspase recruitment domain (CARD). This gene is an intracellular pattern recognition receptor, which is similar in structure to resistant proteins of plants, and mediates innate and acquired immunity by recognizing bacterial molecules containing D-glutamyl-meso-diaminopimelic acid (iE-DAP) moiety. What wore, it has been shown that NOD1 can sense cytosolic microbial products by monitoring the activation state of small Rho GTPases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9294 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat
Immunocytochemistry, 0.5-1μg/ml
Western blot, 0.1-0.5μg/ml
Positive Control
WB: A549 Whole Cell, Rat Cardiac Muscle Tissue
IHC: Human Placenta tissue
ICC: A549 Cell, Hela Cell, SMMC-7721 Cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CARD4 using anti-CARD4 antibody (PB9294).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: A549 Whole Cell Lysate,
Lane 2: Rat Cardiac Muscle Tissue Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CARD4 antigen affinity purified polyclonal antibody (Catalog # PB9294) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CARD4 at approximately 107KD. The expected band size for CARD4 is at 107KD.
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Figure 2. IHC analysis of CARD4 using anti-CARD4 antibody (PB9294).
CARD4 was detected in paraffin-embedded section of Human Placenta Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CARD4 Antibody (PB9294) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of CARD4 using anti-CARD4 antibody (PB9294).
CARD4 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-CARD4 Antibody (PB9294) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of CARD4 using anti-CARD4 antibody (PB9294).
CARD4 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-CARD4 Antibody (PB9294) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of CARD4 using anti-CARD4 antibody (PB9294).
CARD4 was detected in immunocytochemical section of SMMC-7721 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-CARD4 Antibody (PB9294) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For NOD1 (Source: Uniprot.org, NCBI)
Gene Name
NOD1
Full Name
Nucleotide-binding oligomerization domain-containing protein 1
Weight
107691 MW
Alternative Names
Nucleotide-binding oligomerization domain-containing protein 1;Caspase recruitment domain-containing protein 4;NOD1;CARD4; NOD1 CARD4, CLR7.1, NLRC1 nucleotide binding oligomerization domain containing 1 nucleotide-binding oligomerization domain-containing protein 1|NLR family, CARD domain containing 1|caspase recruitment domain family, member 4|caspase recruitment domain-containing protein 4|nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NOD1, check out the NOD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NOD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CARD4/NOD1 Antibody Picoband® (PB9294)
Hello CJ!
PB9294 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ma X,Zhang W,Xu C,Zhang S,Zhao J,Pan Q,Wang Z.Nucleotide-binding oligomerization domain protein 1 enhances oxygen-glucose deprivation and reperfusion injury in cortical neurons via activation of endoplasmic reticulum stress-mediated autophagy. Exp Mol Pat
Species: Rat, Lentivirus
PB9294 usage in article: APP:CO-IMMUNOPRECIPITATION, SAMPLE:PRIMARY RAT CORTICAL NEURON CELLS, DILUTION:1:1000
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Customer Q&As
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6 Customer Q&As for Anti-CARD4/NOD1 Antibody Picoband®
Question
Is a blocking peptide available for product anti-CARD4/NOD1 antibody (PB9294)?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
We do provide the blocking peptide for product anti-CARD4/NOD1 antibody (PB9294). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-05-06
Question
I see that the anti-CARD4/NOD1 antibody PB9294 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-08-29
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-08-29
Question
We are currently using anti-CARD4/NOD1 antibody PB9294 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on pig tissues as well?
W. Banerjee
Verified customer
Asked: 2016-10-04
Answer
The anti-CARD4/NOD1 antibody (PB9294) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-10-04
Question
Please see the WB image, lot number and protocol we used for endothelial cell using anti-CARD4/NOD1 antibody PB9294. Please let me know if you require anything else.
S. Jones
Verified customer
Asked: 2016-07-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-07-01
Question
We need to test anti-CARD4/NOD1 antibody PB9294 on rat endothelial cell for research purposes, then I may be interested in using anti-CARD4/NOD1 antibody PB9294 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Anderson
Verified customer
Asked: 2013-04-08
Answer
The products we sell, including anti-CARD4/NOD1 antibody PB9294, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-04-08
Question
Would anti-CARD4/NOD1 antibody PB9294 work for WB with endothelial cell?
C. Taylor
Verified customer
Asked: 2013-01-10
Answer
According to the expression profile of endothelial cell, NOD1 is highly expressed in endothelial cell. So, it is likely that anti-CARD4/NOD1 antibody PB9294 will work for WB with endothelial cell.
Boster Scientific Support
Answered: 2013-01-10
