Click image to see more details
Product Info Summary
| SKU: | A07686-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CARD10 Antibody
SKU/Catalog Number
A07686-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CARD10 Antibody (Catalog # A07686-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
CARD10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-CARD10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07686-1)
Host
Rabbit
Contents
CARD10 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CARD10 antibody was raised against a synthetic peptide corresponding to 14 amino acids at the C-terminus of human CARD10. The immunogen is located within the last 50 amino acids of CARD10.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
GDF1 antibody is predicted to not cross-react with any other members of the growth differentiation factor family.
Reactive Species
A07686-1 is reactive to CARD10 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
115931 MW
Background of CARD10
Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, Bcl-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD10. This protein belongs to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARD 10 interacts with Bcl-10, a Bcl protein that promotes apoptosis, caspase-9 maturation and activation of NF-κB. CARD 10 and the related protein CARMA1 also associate with NEMO, the regulatory subunit of the IκK complex, demonstrating its importance in the regulation of NF-κB transcription factor activation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07686-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CARD10 antibody can be used for detection of CARD10 by Western blot at 5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of CARD10 in A-20 cell lysate with CARD10 antibody at 5 μg/mL.
Click image to see more details
Immunocytochemistry of CARD10 in EL4 cells with CARD10 antibody at 10 μg/mL.
Click image to see more details
Immunofluorescence of CARD10 in EL4 cells with CARD10 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For CARD10 (Source: Uniprot.org, NCBI)
Gene Name
CARD10
Full Name
Caspase recruitment domain-containing protein 10
Weight
115931 MW
Alternative Names
BIMP1, CARMA3, Caspase recruitment domain-containing protein 10, CARD-containing MAGUK protein 3, Carma 3 CARD10 BIMP1, CARMA3 caspase recruitment domain family member 10 caspase recruitment domain-containing protein 10|Bcl10 binding protein and activator of NFKB|CARD-containing MAGUK 3 protein|CARD-containing MAGUK protein 3|carma 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CARD10, check out the CARD10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CARD10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CARD10 Antibody (A07686-1)
Hello CJ!
No publications found for A07686-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CARD10 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-CARD10 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
