Anti-MUC3 (Mucin 3) Monoclonal Antibody

Product Name

Anti-MUC3 (Mucin 3) Monoclonal Antibody

View all MUC3 Antibodies

SKU/Catalog Number

M08332

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-MUC3 (Mucin 3) Monoclonal Antibody (Catalog # M08332). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-MUC3 (Mucin 3) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08332)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: MUC3/1154

Isotype

IgG2a, kappa

Immunogen

A synthetic peptide of 35 amino acids, SIB35 (C-HSTPSFTSSITTTETTSHSTPSFTSSITTTETTS), which contains two of the MUC3 tandem repeats, coupled to KLH.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M08332 is reactive to MUC3A in Human

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

345127 MW

Background of MUC3

This monoclonal antibody recognizes a protein of HMW, identified as mucin 3 glycoprotein (MUC3). This monoclonal antibody shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC2. The Mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major component of various mucus gels and is broadly expressed in normal and tumor cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M08332 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For MUC3A (Source: Uniprot.org, NCBI)

Alternative Names

Mucin-3A ;MUC-3A ;Intestinal mucin-3A ;MUC3A ;MUC3 ; MUC3A MUC-3A, MUC3 mucin 3A, cell surface associated mucin-3A|intestinal mucin|mucin 3, intestinal

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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