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Product Info Summary
| SKU: | A07686 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CARD 10 Antibody
SKU/Catalog Number
A07686
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CARD 10 Antibody catalog # A07686. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CARD 10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07686)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human CARD10. AA range:481-530
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07686 is reactive to CARD10 in Human, Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
115931 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07686 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using CARD 10 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For CARD10 (Source: Uniprot.org, NCBI)
Gene Name
CARD10
Full Name
Caspase recruitment domain-containing protein 10
Weight
115931 MW
Alternative Names
Caspase recruitment domain-containing protein 10;CARD-containing MAGUK protein 3;Carma 3;CARD10;CARMA3; CARD10 BIMP1, CARMA3 caspase recruitment domain family member 10 caspase recruitment domain-containing protein 10|Bcl10 binding protein and activator of NFKB|CARD-containing MAGUK 3 protein|CARD-containing MAGUK protein 3|carma 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CARD10, check out the CARD10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CARD10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CARD 10 Antibody (A07686)
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No publications found for A07686
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Customer Q&As
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5 Customer Q&As for Anti-CARD 10 Antibody
Question
We are currently using anti-CARD 10 antibody A07686 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2017-11-14
Answer
The anti-CARD 10 antibody (A07686) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-14
Question
Does anti-CARD 10 antibody A07686 work on horse WB with parotid gland?
Verified Customer
Verified customer
Asked: 2017-06-29
Answer
Our lab technicians have not validated anti-CARD 10 antibody A07686 on horse. You can run a BLAST between horse and the immunogen sequence of anti-CARD 10 antibody A07686 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse parotid gland in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-29
Question
Does A07686 anti-CARD 10 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Li
Verified customer
Asked: 2015-05-13
Answer
As indicated on the product datasheet, A07686 anti-CARD 10 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-05-13
Question
I was wanting to use your anti-CARD 10 antibody for WB for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
S. Dhar
Verified customer
Asked: 2013-12-26
Answer
It shows on the product datasheet, A07686 anti-CARD 10 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-12-26
Question
I see that the anti-CARD 10 antibody A07686 works with WB, what is the protocol used to produce the result images on the product page?
T. Taylor
Verified customer
Asked: 2013-08-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-08-06
