Anti-CARD 10 Antibody

CARD10 antibody

Boster Bio Anti-CARD 10 Antibody catalog # A07686. Tested in WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A07686
Size: 100μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

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Product Name

Anti-CARD 10 Antibody

View all CARD10 Antibodies

SKU/Catalog Number

A07686

Size

100μl

Form

Liquid

Description

Boster Bio Anti-CARD 10 Antibody catalog # A07686. Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CARD 10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07686)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human CARD 10. at AA rangle: 450-530

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07686 is reactive to CARD10 in Human, Mouse

Applications

A07686 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

115931 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For CARD10 (Source: Uniprot.org, NCBI)

Gene Name

CARD10

Full Name

Caspase recruitment domain-containing protein 10

Weight

115931 MW

Alternative Names

CARD-containing MAGUK 3 protein; CARD-containing MAGUK protein 3; Carma 3; CARMA3BIMP1Bcl10 binding protein and activator of NFKB; caspase recruitment domain family, member 10; caspase recruitment domain-containing protein 10; MGC142219 CARD10 BIMP1, CARMA3 caspase recruitment domain family member 10 caspase recruitment domain-containing protein 10|Bcl10 binding protein and activator of NFKB|CARD-containing MAGUK 3 protein|CARD-containing MAGUK protein 3|carma 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CARD10, check out the CARD10 Infographic

CARD10 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CARD10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-CARD 10 Antibody

Question

We are currently using anti-CARD 10 antibody A07686 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2017-11-14

Answer

The anti-CARD 10 antibody (A07686) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-11-14

Question

Does anti-CARD 10 antibody A07686 work on horse WB with parotid gland?

Verified Customer

Verified customer

Asked: 2017-06-29

Answer

Our lab technicians have not validated anti-CARD 10 antibody A07686 on horse. You can run a BLAST between horse and the immunogen sequence of anti-CARD 10 antibody A07686 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse parotid gland in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-06-29

Question

Does A07686 anti-CARD 10 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

J. Li

Verified customer

Asked: 2015-05-13

Answer

As indicated on the product datasheet, A07686 anti-CARD 10 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2015-05-13

Question

I was wanting to use your anti-CARD 10 antibody for WB for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?

S. Dhar

Verified customer

Asked: 2013-12-26

Answer

It shows on the product datasheet, A07686 anti-CARD 10 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-12-26

Question

I see that the anti-CARD 10 antibody A07686 works with WB, what is the protocol used to produce the result images on the product page?

T. Taylor

Verified customer

Asked: 2013-08-06

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2013-08-06

Order DetailsPrice
A07686

100uL

$399

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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