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Product Info Summary
| SKU: | M08065-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Calponin CNN1 Rabbit Monoclonal Antibody
View all Calponin 1 Antibodies
SKU/Catalog Number
M08065-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Calponin CNN1 Rabbit Monoclonal Antibody catalog # M08065-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Calponin CNN1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08065-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BCC-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Calponin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M08065-1 is reactive to CNN1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
35 kDa
Calculated molecular weight
33170 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08065-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
IHC: human appendicitis tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CNN1 using anti-CNN1 antibody (M08065-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human U20S whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: mouse stomach tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CNN1 antigen affinity purified monoclonal antibody (Catalog # M08065-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CNN1 at approximately 35 kDa. The expected band size for CNN1 is at 33 kDa.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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IHC analysis of Calponin using anti-Calponin antibody (M08065-1).
Calponin was detected in a paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Calponin Antibody (M08065-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CNN1 (Source: Uniprot.org, NCBI)
Gene Name
CNN1
Full Name
Calponin-1
Weight
33170 MW
Superfamily
calponin family
Alternative Names
Calponin-1;Basic calponin;Calponin H1, smooth muscle;CNN1; CNN1 HEL-S-14, SMCC, Sm-Calp calponin 1 calponin-1|basic calponin|calponin 1, basic, smooth muscle|calponin H1, smooth muscle|calponins, basic|epididymis secretory protein Li 14
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CNN1, check out the CNN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CNN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Calponin CNN1 Rabbit Monoclonal Antibody (M08065-1)
Hello CJ!
M08065-1 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li X,Hu B,Wang L,Xia Q,Ni X. P2X7 receptor-mediated phenotype switching of pulmonary artery smooth muscle cells in hypoxia. Mol Biol Rep.2021 Mar 1.doi:10.1007/s11033-021-06222-2. Epub ahead of print.PMID:33650080.
Species: Rat
M08065-1 usage in article: APP:WB, SAMPLE:PASMCS, DILUTION:1:500
Zhao Y,Tang N,Xi D,Huang Z,Zhang T,Liu Y,Wang L,Tang Y,Zhong H,He F.Calcilytic NPS2143 promotes proliferation and inhibits apoptosis of spontaneously hypertensive rat vascular smooth muscle cells via activation of the renin-angiotensin system.Exp Ther Med
Species: Rat
M08065-1 usage in article: APP:WB, SAMPLE:VSMCS, DILUTION:1:400
Functional and Proteomic Investigations Reveal Major Royal Jelly Protein 1 Associated with Anti-hypertension Activity in Mouse Vascular Smooth MuscleCells
Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated with Activation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension
Effects of BMSCs interactions with adventitial fibroblasts in transdifferentiation and ultrastructure processes
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