This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
7 Citations 1 Q&As
2 Citations 3 Q&As
5 Citations
Facts about Calponin-1.
The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity). .
Human | |
---|---|
Gene Name: | CNN1 |
Uniprot: | P51911 |
Entrez: | 1264 |
Belongs to: |
---|
calponin family |
Basic calponin; Calponin 1; calponin 1, basic, smooth muscle; Calponin H1; Calponin H1, smooth muscle; calponins, basic; CNN1; Sm-Calp; SMCC; SMCCcalponin-1
Mass (kDA):
33.17 kDA
Human | |
---|---|
Location: | 19p13.2 |
Sequence: | 19; NC_000019.10 (11538775..11550323) |
Smooth muscle, and tissues containing significant amounts of smooth muscle.
Boster Bio smooth muscular marker monoclonal antibodies are a great way to improve your lab workflow. This antibody offers many features that will make your research more efficient. Researchers can submit their findings for species, applications, or special samples. By sharing their results, scientists can receive product credits. Boster Bio products can be used by scientists around the world. You can also earn credits for sharing your results.
Boster Bio is a company that produces high-quality antibodies and ELISA kits for research on cancer, neuroscience, inflammation, and developmental biology. Their antibodies are validated for use in WB and ELISA and come with Boster's Boster Quality Guarantee. Boster Biologicals' primary and secondary antibodies against CNN1 are aimed at the neuroscience community. They can be used in a variety of applications.
Boster's neuroscience, neuroendocrine and immunological antibodies are used by researchers all over the world to study brain function, immune function, and other tissues. The company manufactures monoclonal antibodies for use in IHC staining, and offers over 4,000 research antibodies and ELISA kits. Researchers can also select from the company’s collection of research products including antibodies to cardiovascular and viral markers.
Boster Bio stocks monoclonal antibodies from rabbit and goat, as well research-grade polyclonal antibodies. Boster Bio also offers a variety of biodiagnostic products including primary and secondary antibodies against CNN1. Their product offerings include reagents for cell separation and purification, isotype controls, and a variety of recombinant antibodies.
Primary and secondary antibodies against the CNN1 marker from Boster Bio are excellent options for use in immunocytochemistry. They are extremely sensitive and should not have been used in immunocytochemistry without being tested in a titration circle to ensure that they bind the target protein. They can also be used to immunocytochemistry since they bind with the CNN1 protein.
Primary antibody control is derived by transfected cell-line lines that express antigen protein. The conditions for fixing and diluting primary antibody are the same as those for primary antibodies. The untransfected cells serve as negative controls, not expressing the protein of interest. SiRNA can also be used to reduce the expression of the antigen protein within the cell line.
Boster Bio is an internationally recognized company that develops antibody and ELISA kits for the detection of many biomarkers from developmental biology to neurosciences and inflammation. Their kits are highly sensitive and reach the picogram level of sensitivity. Boster Bio's CNN1 Research ELISA Kit is a great choice for researchers who want to measure the protein levels in a variety samples.
To detect Calponin-1, we used the smooth muscle marker monocleosome from Boster Bio. This antibody reacts with Calponin-1 protein in immunoblotting experiments. The antibody also labels smooth muscle cells in paraffin embedded tissue sections frozen or formalin-fixed. The antibodies were used to study the function and structure of smooth muscle in various tissues.
We performed several experiments to determine the function of this antigen. CCD-18Co cells were cultured on coverslips in 12-well plates and seeded at 5000 cells/cm2. After fixing the cells with 4% formaldehyde we permeabilized them with 0.1% Triton X-100 and then blocked them with 3% BSA. Next, we incubated them with a rabbit anti-COL1A1 polyclonal antibody. We also used a mouse monoclonal anti-COL1A1 antibody purchased from OriGene.
Another important feature of this anti-actin antibody is its ability to detect human alpha-Smooth Muscle Actin in a variety of cell types, including endothelial, cellular, and nerve cells. The antibodies do not detect the protein within other cell types like fibroblasts, endothelium and striated muscles.
Boster Bio's smooth-muscle marker monoconjugate, which is made from smooth muscle markers, is also an important tool in research in the planarian field. Its antisynaptin or anti-SMEDWI antibody can be used to perform whole-mount, in situ hybridization tests. This product can detect a wide range of proteins in the body, which is quite remarkable. Its ability to detect these proteins is encouraging.
PMID: 8526917 by Maguchi M., et al. Molecular cloning and gene mapping of human basic and acidic calponins.
PMID: 9332369 by Miano J.M., et al. Expression, genomic structure and high resolution mapping to 19p13.2 of the human smooth muscle cell calponin gene.
*More publications can be found for each product on its corresponding product page