Anti-Calponin-1 (Smooth Muscle Marker) Monoclonal Antibody

Product Name

Anti-Calponin-1 (Smooth Muscle Marker) Monoclonal Antibody

View all Calponin 1 Antibodies

SKU/Catalog Number

M08065

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Calponin-1 (Smooth Muscle Marker) Monoclonal Antibody (Catalog # M08065). Tested in WB, Flow Cytometry, IHC applications. This antibody reacts with Human, Rat.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Calponin-1 (Smooth Muscle Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08065)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: rCNN1/832

Isotype

IgG1, kappa

Immunogen

Recombinant full-length human CNN1 protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M08065 is reactive to CNN1 in Human, Rat

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

33170 MW

Background of Calponin 1

Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, This monoclonal antibody reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M08065 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For CNN1 (Source: Uniprot.org, NCBI)

Alternative Names

Calponin-1;Basic calponin;Calponin H1, smooth muscle;CNN1; CNN1 HEL-S-14, SMCC, Sm-Calp calponin 1 calponin-1|basic calponin|calponin 1, basic, smooth muscle|calponin H1, smooth muscle|calponins, basic|epididymis secretory protein Li 14

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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