Product Info Summary
SKU: | A03047 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Calbindin/CALB1 Antibody Picoband®
View all Calbindin D-28K Antibodies
SKU/Catalog Number
A03047
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Calbindin/CALB1 Antibody Picoband® catalog # A03047. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Calbindin/CALB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03047)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Calbindin recombinant protein (Position: A2-N261).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03047 is reactive to CALB1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
30 kDa
Calculated molecular weight
30,025 MW
Background of Calbindin D-28K
Calbindin is a calcium-binding protein belonging to the troponin C superfamily. And it mapped to 8q21.3. Calretinin is expressed in central and peripheral nervous system and in many normal and pathological tissues. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Two calcium binding proteins, calbindin and calretinin, have been reported to be expressed in abundance in Purkinje cells and other cell types in the cerebellum.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03047 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Immunofluorescence, 5 μg/ml, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Positive Control
WB: rat brain tissue, rat kidney tissue, mouse brain tissue, mouse kidney tissue
IHC: rat kidney tissue, mouse brain tissue, mouse kidney tissue
IF: rat cerebellum tissue, rat cerebellum tissue
FCM: A431 cell
Validation Images & Assay Conditions
![a03047 calbindin primary antibodies wb testing 1 a03047 calbindin primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a03047-calbindin-primary-antibodies-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blot analysis of Calbindin using anti-Calbindin antibody (A03047).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calbindin antigen affinity purified polyclonal antibody (Catalog # A03047) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calbindin at approximately 30 kDa. The expected band size for Calbindin is at 30 kDa.
![A03047 Calbindin primary antibodies IHC testing 2 A03047 Calbindin primary antibodies IHC testing 2](https://www.bosterbio.com/media/catalog/product/A/0/A03047-Calbindin-primary-antibodies-IHC-testing-2.jpg)
Click image to see more details
Figure 2. IHC analysis of Calbindin using anti-Calbindin antibody (A03047).
Calbindin was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Calbindin Antibody (A03047) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
![A03047 Calbindin primary antibodies IHC testing 3 A03047 Calbindin primary antibodies IHC testing 3](https://www.bosterbio.com/media/catalog/product/A/0/A03047-Calbindin-primary-antibodies-IHC-testing-3.jpg)
Click image to see more details
Figure 3. IHC analysis of Calbindin using anti-Calbindin antibody (A03047).
Calbindin was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Calbindin Antibody (A03047) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
![A03047 Calbindin primary antibodies IHC testing 4 A03047 Calbindin primary antibodies IHC testing 4](https://www.bosterbio.com/media/catalog/product/A/0/A03047-Calbindin-primary-antibodies-IHC-testing-4.jpg)
Click image to see more details
Figure 4. IHC analysis of Calbindin using anti-Calbindin antibody (A03047).
Calbindin was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Calbindin Antibody (A03047) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
![A03047 Calbindin primary antibodies FC testing 5 A03047 Calbindin primary antibodies FC testing 5](https://www.bosterbio.com/media/catalog/product/A/0/A03047-Calbindin-primary-antibodies-FC-testing-5.jpg)
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Figure 5. Flow Cytometry analysis of A431 cells using anti-Calbindin antibody (A03047).
Overlay histogram showing A431 cells stained with A03047 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calbindin Antibody (A03047,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
![a03047 calbindin primary antibodies if testing 6 a03047 calbindin primary antibodies if testing 6](https://www.bosterbio.com/media/catalog/product/a/0/a03047-calbindin-primary-antibodies-if-testing-6.jpg)
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Figure 6. IF analysis of Calbindin using anti-Calbindin antibody (A03047).
Calbindin was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Calbindin Antibody (A03047) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a03047 calbindin primary antibodies if testing 7 a03047 calbindin primary antibodies if testing 7](https://www.bosterbio.com/media/catalog/product/a/0/a03047-calbindin-primary-antibodies-if-testing-7.jpg)
Click image to see more details
Figure 7. IF analysis of Calbindin using anti-Calbindin antibody (A03047).
Calbindin was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Calbindin Antibody (A03047) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For CALB1 (Source: Uniprot.org, NCBI)
Gene Name
CALB1
Full Name
Calbindin
Weight
30,025 MW
Superfamily
calbindin family
Alternative Names
Calbindin; Calbindin D28; D-28K; Vitamin D-dependent calcium-binding protein, avian-type; CALB1; CAB27 CALB1 CALB, D-28K calbindin 1 calbindin|RTVL-H protein|calbindin 1, (28kD)|calbindin 1, 28kDa|calbindin 27|calbindin D28|vitamin D-dependent calcium-binding protein, avian-type
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CALB1, check out the CALB1 Infographic
![CALB1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CALB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Calbindin/CALB1 Antibody Picoband® (A03047)
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Customer Q&As
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15 Customer Q&As for Anti-Calbindin/CALB1 Antibody Picoband®
Question
We have been able to see staining in mouse cerebellum substantia nigra. Are there any suggestions? Is anti-Calbindin/CALB1 antibody supposed to stain cerebellum substantia nigra positively?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
From what I have seen in literature cerebellum substantia nigra does express CALB1. From what I have seen in Uniprot.org, CALB1 is expressed in caudate nucleus, brain, amygdala, cerebellum substantia nigra, kidney lung, among other tissues. Regarding which tissues have CALB1 expression, here are a few articles citing expression in various tissues:
Amygdala, Cerebellum, and Substantia nigra, Pubmed ID: 14702039
Brain, Pubmed ID: 2565876, 3691519
Kidney, and Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-03-31
Question
Is a blocking peptide available for product anti-Calbindin/CALB1 antibody (A03047)?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
We do provide the blocking peptide for product anti-Calbindin/CALB1 antibody (A03047). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-11
Question
Is there a BSA free version of anti-Calbindin/CALB1 antibody A03047 available?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Calbindin/CALB1 antibody A03047 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-22
Question
My question regards to test anti-Calbindin/CALB1 antibody A03047 on human brain for research purposes, then I may be interested in using anti-Calbindin/CALB1 antibody A03047 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
The products we sell, including anti-Calbindin/CALB1 antibody A03047, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-30
Question
I was wanting to use your anti-Calbindin/CALB1 antibody for ELISA for human brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
It shows on the product datasheet, A03047 anti-Calbindin/CALB1 antibody has been tested for ELISA, Flow Cytometry, IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-10
Question
Does anti-Calbindin/CALB1 antibody A03047 work for ELISA with brain?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
According to the expression profile of brain, CALB1 is highly expressed in brain. So, it is likely that anti-Calbindin/CALB1 antibody A03047 will work for ELISA with brain.
Boster Scientific Support
Answered: 2019-07-03
Question
My question regarding product A03047, anti-Calbindin/CALB1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-10-23
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03047 anti-Calbindin/CALB1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-23
Question
Is this A03047 anti-Calbindin/CALB1 antibody reactive to the isotypes of CALB1?
J. Anderson
Verified customer
Asked: 2016-02-15
Answer
The immunogen of A03047 anti-Calbindin/CALB1 antibody is E. coli-derived human Calbindin recombinant protein (Position: A2-N261). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-02-15
Question
I am interested in using your anti-Calbindin/CALB1 antibody for metanephric collecting duct development studies. Has this antibody been tested with western blotting on a431 cells? We would like to see some validation images before ordering.
J. Mitchell
Verified customer
Asked: 2015-12-31
Answer
I appreciate your inquiry. This A03047 anti-Calbindin/CALB1 antibody is validated on rat kidney tissue, brain tissue, mouse kidney tissue, a431 cells. It is guaranteed to work for ELISA, Flow Cytometry, IHC-P, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-12-31
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Calbindin/CALB1 antibody A03047. Let me know if you need anything else.
S. Huang
Verified customer
Asked: 2015-12-02
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-12-02
Question
We are currently using anti-Calbindin/CALB1 antibody A03047 for human tissue, and we are happy with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
W. Singh
Verified customer
Asked: 2015-11-30
Answer
The anti-Calbindin/CALB1 antibody (A03047) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-11-30
Question
See below the WB image, lot number and protocol we used for brain using anti-Calbindin/CALB1 antibody A03047. Please let me know if you require anything else.
H. Huang
Verified customer
Asked: 2015-03-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-03-13
Question
We ordered your anti-Calbindin/CALB1 antibody for ELISA on cerebellum substantia nigra a few years ago. I am using rat, and I plan to use the antibody for Flow Cytometry next. I am interested in examining cerebellum substantia nigra as well as amygdala in our next experiment. Could give a recommendation on which antibody would work the best for Flow Cytometry?
R. Patel
Verified customer
Asked: 2015-01-06
Answer
I took a look at the website and datasheets of our anti-Calbindin/CALB1 antibody and it seems that A03047 has been validated on rat in both ELISA and Flow Cytometry. Thus A03047 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-01-06
Question
Will A03047 anti-Calbindin/CALB1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Carter
Verified customer
Asked: 2014-03-12
Answer
You can see on the product datasheet, A03047 anti-Calbindin/CALB1 antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-03-12
Question
I see that the anti-Calbindin/CALB1 antibody A03047 works with ELISA, what is the protocol used to produce the result images on the product page?
C. Yang
Verified customer
Asked: 2014-03-04
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-03-04