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Product Info Summary
| SKU: | M03047-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Bovine, Human, Mouse, Pig, Rat |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Calbindin Monoclonal Antibody
View all Calbindin D-28K Antibodies
SKU/Catalog Number
M03047-1
Size
100ul
Description
Boster Bio Anti-Calbindin Monoclonal Antibody catalog # M03047-1. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Pig, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Calbindin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03047-1)
Host
Mouse
Contents
Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Monoclonal
Immunogen
Full-length recombinant human calbindin protein expressed in and purified from E.Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03047-1 is reactive to CALB1 in Bovine, Human, Mouse, Pig, Rat
Reconstitution
Calculated molecular weight
30025 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03047-1 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:5,000. ICC/IF or IHC: 1:1,000.
Validation Images & Assay Conditions
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Western blot analysis of different neuronal tissue lysates using mouse mAb to calbindin, M03047-1, dilution 1:2,000: [1] protein standard,
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Immunofluorescent analysis of rat brain cerebellum section stained with mouse mAb to calbindin, M03047-1, dilution 1:1,000, in green, and costained with rabbit pAb to calretinin, dilution 1:5,000 in red. The blue is DAPI staining of nuclear DNA Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45M, and free-floating sections were stained with the above antibodies. Calbindin antibody prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of cerebellum. In contrast the calretinin antibody stains granule cells, in the granualar layer, and their processes in the molecular layer.
Protein Target Info & Infographic
Gene/Protein Information For CALB1 (Source: Uniprot.org, NCBI)
Gene Name
CALB1
Full Name
Calbindin
Weight
30025 MW
Superfamily
calbindin family
Alternative Names
Calbindin;Calbindin D28;D-28K;Vitamin D-dependent calcium-binding protein, avian-type;CALB1;CAB27; CALB1 CALB, D-28K calbindin 1 calbindin|RTVL-H protein|calbindin 1, (28kD)|calbindin 1, 28kDa|calbindin 27|calbindin D28|vitamin D-dependent calcium-binding protein, avian-type
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CALB1, check out the CALB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CALB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Calbindin Monoclonal Antibody (M03047-1)
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No publications found for M03047-1
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Customer Q&As
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15 Customer Q&As for Anti-Calbindin Monoclonal Antibody
Question
Is this M03047-1 anti-Calbindin Monoclonal antibody reactive to the isotypes of CALB1?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
The immunogen of M03047-1 anti-Calbindin Monoclonal antibody is Full-length recombinant human calbindin protein expressed in and purified from E.Coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-26
Question
We have observed staining in rat caudate nucleus. Are there any suggestions? Is anti-Calbindin Monoclonal antibody supposed to stain caudate nucleus positively?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
From literature caudate nucleus does express CALB1. From Uniprot.org, CALB1 is expressed in caudate nucleus, brain, amygdala, cerebellum substantia nigra, kidney lung, among other tissues. Regarding which tissues have CALB1 expression, here are a few articles citing expression in various tissues:
Amygdala, Cerebellum, and Substantia nigra, Pubmed ID: 14702039
Brain, Pubmed ID: 2565876, 3691519
Kidney, and Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-10-30
Question
See below the WB image, lot number and protocol we used for amygdala using anti-Calbindin Monoclonal antibody M03047-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-17
Question
Can you help my question with product M03047-1, anti-Calbindin Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03047-1 anti-Calbindin Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-31
Question
We want to test anti-Calbindin Monoclonal antibody M03047-1 on human amygdala for research purposes, then I may be interested in using anti-Calbindin Monoclonal antibody M03047-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
The products we sell, including anti-Calbindin Monoclonal antibody M03047-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-28
Question
Our lab used your anti-Calbindin Monoclonal antibody for WB on cerebellum substantia nigra in a previous project. I am using bovine, and We want to use the antibody for IHC next. My lab would like examining cerebellum substantia nigra as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2018-09-04
Answer
I have checked the website and datasheets of our anti-Calbindin Monoclonal antibody and it appears that M03047-1 has been validated on bovine in both WB and IHC. Thus M03047-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in bovine even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-09-04
Question
I am looking for using your anti-Calbindin Monoclonal antibody for amyloid fiber formation studies. Has this antibody been tested with western blotting on neuronal tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-07-16
Answer
Thanks for your inquiry. This M03047-1 anti-Calbindin Monoclonal antibody is tested on neuronal tissue, purkinje cells, granule cells. It is guaranteed to work for IF, IHC, WB in bovine, human, mouse, pig, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-07-16
Question
We are currently using anti-Calbindin Monoclonal antibody M03047-1 for rat tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says bovine, human, mouse, pig, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-04
Answer
The anti-Calbindin Monoclonal antibody (M03047-1) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-04
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for amygdala using anti-Calbindin Monoclonal antibody M03047-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-10-04
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-04
Question
Would M03047-1 anti-Calbindin Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
F. Krishna
Verified customer
Asked: 2017-08-11
Answer
It shows on the product datasheet, M03047-1 anti-Calbindin Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-08-11
Question
Would anti-Calbindin Monoclonal antibody M03047-1 work for IHC with amygdala?
Verified Customer
Verified customer
Asked: 2017-06-08
Answer
According to the expression profile of amygdala, CALB1 is highly expressed in amygdala. So, it is likely that anti-Calbindin Monoclonal antibody M03047-1 will work for IHC with amygdala.
Boster Scientific Support
Answered: 2017-06-08
Question
I was wanting to use your anti-Calbindin Monoclonal antibody for IHC for human amygdala on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human amygdala identification?
Verified Customer
Verified customer
Asked: 2017-06-05
Answer
You can see on the product datasheet, M03047-1 anti-Calbindin Monoclonal antibody has been validated for IF, IHC, WB on bovine, human, mouse, pig, rat tissues. We have an innovator award program that if you test this antibody and show it works in human amygdala in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-05
Question
Is a blocking peptide available for product anti-Calbindin Monoclonal antibody (M03047-1)?
K. Singh
Verified customer
Asked: 2016-10-14
Answer
We do provide the blocking peptide for product anti-Calbindin Monoclonal antibody (M03047-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-10-14
Question
I see that the anti-Calbindin Monoclonal antibody M03047-1 works with IHC, what is the protocol used to produce the result images on the product page?
N. Krishna
Verified customer
Asked: 2013-04-10
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-04-10
Question
Do you have a BSA free version of anti-Calbindin Monoclonal antibody M03047-1 available?
P. Walker
Verified customer
Asked: 2013-03-05
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Calbindin Monoclonal antibody M03047-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-03-05
