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Product Info Summary
| SKU: | M03047-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Bovine, Human, Mouse, Rat |
| Host: | Chicken |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Calbindin Antibody
View all Calbindin D-28K Antibodies
SKU/Catalog Number
M03047-2
Size
100ul
Description
Boster Bio Anti-Calbindin Antibody catalog # M03047-2. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Calbindin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03047-2)
Host
Chicken
Contents
Supplied as an aliquot of IgY preparation plus 5mM NaN3
Clonality
Polyclonal
Immunogen
Full-length recombinant human protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03047-2 is reactive to CALB1 in Bovine, Human, Mouse, Rat
Reconstitution
Calculated molecular weight
30025 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03047-2 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:5,000. IF/ICC or IHC: 1:1,000-1:5,000.
Validation Images & Assay Conditions
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Western blot analysis of different tissue lysates and recombinant protein solutions using chicken pAb to calbindin, M03047-2, dilution 1:5,000 in green: [1] protein standard (red), [2] rat cerebellum, [3] pig hippocampus, [4] cow cerebellum, [5] protein standard (red). Next lanes are full length recombinant human proteins, [6] Parvalbumin, [7] Calretinin, [8] Calbindin. Bands at 25kDa in tissue lysates and ~30 kDa in protein solutions correspond to calbindin, the recombinant form being slightly larger due to the presence of a His tag and other vector derived sequence. The M03047-2 antibody specifically recognizes calbindin protein and does not react with the closely related proteins parvalbumin and calretinin.
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Immunofluorescent analysis of rat cerebellum section stained with chicken pAb to calbindin, M03047-2, dilution 1:2,000, in green, and costained with rabbit pAb to MeCP2, dilution 1:5,000, in red. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45M, and free-floating sections were stained with above antibodies. Calbindin, often used as a Purkinje cell marker, is prominently detected in dendrites and perikarya of these cells in the cerebellar molecular layer. The MeCP2 antibody selectively stains nuclei of neuronal cells.
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Figure 3. IHC analysis of calbindin using anti-calbindin antibody (M03047-2).
Calbindin was detected in a mice cerebellum. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-calbindin antibody (M03047-2) at 1:250 overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody at 1:250 and incubated for 2h at room temperature. The tissue section was developed using fluorescence.
Protein Target Info & Infographic
Gene/Protein Information For CALB1 (Source: Uniprot.org, NCBI)
Gene Name
CALB1
Full Name
Calbindin
Weight
30025 MW
Superfamily
calbindin family
Alternative Names
Calbindin;Calbindin D28;D-28K;Vitamin D-dependent calcium-binding protein, avian-type;CALB1;CAB27; CALB1 CALB, D-28K calbindin 1 calbindin|RTVL-H protein|calbindin 1, (28kD)|calbindin 1, 28kDa|calbindin 27|calbindin D28|vitamin D-dependent calcium-binding protein, avian-type
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CALB1, check out the CALB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CALB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Calbindin Antibody (M03047-2)
Hello CJ!
M03047-2 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Inappropriate Intrusion of an Axonal Mitochondrial Anchor into Dendrites Causes Neurodegeneration
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Customer Reviews
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1 Reviews For Anti-Calbindin Antibody
0
Best Calbindin Antibody for IHC in Mouse Cerebellum--Dinesh Joshi, University of Wisconsin Madison, Research Scientist
Excellent
Source: Biocompare.com
| Application | Immunohistochemistry |
|---|---|
| Sample | Mice Cerebellum |
| Detection | Fluorescence |
"I tried this antibody for Purkinje cell labeling in mice cerebellum using IHC-P. Antigen retrieval - 20min boiling in EDTA based buffer. Permeabilization - 0.3% triton for 15min. Blocking - 10% goat serum for 1hr. Primary antibody - 1:250 overnight at 4 Degree C. Secondary antibody - Fluorescence conjugated secondary antibody at 1:250 for 2hr at RT. I was amazed to see the beautiful dendrites, soma and axons. In my hand, this is the best labeling of Purkinje cells I have ever seen using IHC-P. Beautiful labeling of Purkinje cell soma, dendrites and axons. Best antibody I have used till now. Highly recommended for IHC-P."
Dinesh Joshi
Verified customer
Submitted 2019-12-06
Customer Q&As
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Find answers in Q&As, reviews.
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15 Customer Q&As for Anti-Calbindin Antibody
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney lung using anti-Calbindin antibody M03047-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-29
Question
We want to test anti-Calbindin antibody M03047-2 on bovine kidney lung for research purposes, then I may be interested in using anti-Calbindin antibody M03047-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Jackson
Verified customer
Asked: 2019-11-28
Answer
The products we sell, including anti-Calbindin antibody M03047-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-28
Question
Will M03047-2 anti-Calbindin antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
As indicated on the product datasheet, M03047-2 anti-Calbindin antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-14
Question
See below the WB image, lot number and protocol we used for kidney lung using anti-Calbindin antibody M03047-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-08
Question
We have seen staining in human kidney lung. What should we do? Is anti-Calbindin antibody supposed to stain kidney lung positively?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
Based on literature kidney lung does express CALB1. Based on Uniprot.org, CALB1 is expressed in caudate nucleus, brain, amygdala, cerebellum substantia nigra, kidney lung, among other tissues. Regarding which tissues have CALB1 expression, here are a few articles citing expression in various tissues:
Amygdala, Cerebellum, and Substantia nigra, Pubmed ID: 14702039
Brain, Pubmed ID: 2565876, 3691519
Kidney, and Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-09-27
Question
I was wanting to use your anti-Calbindin antibody for IF for bovine kidney lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for bovine kidney lung identification?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
It shows on the product datasheet, M03047-2 anti-Calbindin antibody has been validated for IF, IHC, WB on bovine, human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in bovine kidney lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-23
Question
Is there a BSA free version of anti-Calbindin antibody M03047-2 available?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Calbindin antibody M03047-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-06
Question
you antibody using your anti-Calbindin antibody for retina layer formation studies. Has this antibody been tested with western blotting on neuronal cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
We appreciate your inquiry. This M03047-2 anti-Calbindin antibody is validated on neuronal cells. It is guaranteed to work for IF, IHC, WB in bovine, human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-07-03
Question
I see that the anti-Calbindin antibody M03047-2 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-01-21
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-01-21
Question
Is this M03047-2 anti-Calbindin antibody reactive to the isotypes of CALB1?
Verified Customer
Verified customer
Asked: 2018-11-30
Answer
The immunogen of M03047-2 anti-Calbindin antibody is Full-length recombinant human protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-11-30
Question
I have a question about product M03047-2, anti-Calbindin antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-01-12
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03047-2 anti-Calbindin antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-12
Question
Is a blocking peptide available for product anti-Calbindin antibody (M03047-2)?
Verified Customer
Verified customer
Asked: 2017-10-20
Answer
We do provide the blocking peptide for product anti-Calbindin antibody (M03047-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-10-20
Question
We are currently using anti-Calbindin antibody M03047-2 for bovine tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says bovine, human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-28
Answer
The anti-Calbindin antibody (M03047-2) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-28
Question
Does anti-Calbindin antibody M03047-2 work for IF with kidney lung?
S. Rodriguez
Verified customer
Asked: 2016-02-18
Answer
According to the expression profile of kidney lung, CALB1 is highly expressed in kidney lung. So, it is likely that anti-Calbindin antibody M03047-2 will work for IF with kidney lung.
Boster Scientific Support
Answered: 2016-02-18
Question
We ordered your anti-Calbindin antibody for IF on brain a few years ago. I am using bovine, and We intend to use the antibody for WB next. I am interested in examining brain as well as amygdala in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
J. Jones
Verified customer
Asked: 2013-12-23
Answer
I looked at the website and datasheets of our anti-Calbindin antibody and I see that M03047-2 has been validated on bovine in both IF and WB. Thus M03047-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in bovine even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-12-23
