Click image to see more details
-
-
-
-
-
+3
Product Info Summary
| SKU: | MA1007 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Bovine, Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Calbindin-D Calb1 Antibody (Monoclonal, CB-955)
View all Calbindin D-28K Antibodies
SKU/Catalog Number
MA1007
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Calbindin-D Calb1 Antibody (Monoclonal, CB-955) catalog # MA1007. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Calbindin-D Calb1 Antibody (Monoclonal, CB-955) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1007)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
CB-955
Isotype
Mouse IgG1
Immunogen
Bovine kidney calbindin-D
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1007 is reactive to Calb1 in Bovine, Human, Mouse, Rat
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Observed Molecular Weight
28 kDa
Calculated molecular weight
29994 MW
Background of Calbindin D-28K
The human calbindin D28k (CALB1) is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. Parmentier et al. (1991) mapped the CALB1 gene to 8q21.3-q22.1 by in situ hybridization.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1007 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, bovine, mouse, rat, By Heat
Western blot, 0.5-1μg/ml, Human, bovine, mouse, rat
Immunofluorescence, 2μg/ml, Mouse
Positive Control
WB: Rat Brain Tissue, Rat Brain Tissue, Rat Kidney Tissue, Rat Lung Tissue, rat brain tissue, rat kidney tissue, mouse brain tissue
IHC: Mouse Kidney Tissue, Human Intestinal Cancer Tissue, Rat Kidney Tissue, Human Mammary Cancer Tissue
IF: mouse brain tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-Calbindin-D antibody (monoclonal), MA1007, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Kidney Tissue Lysate
Lane 4: Rat Lung Tissue Lysate
Click image to see more details
Anti-Calbindin-D antibody (monoclonal), MA1007, IHC(P)
IHC(P): Mouse Kidney Tissue
Click image to see more details
Anti-Calbindin-D antibody (monoclonal), MA1007, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Click image to see more details
Anti-Calbindin-D antibody (monoclonal), MA1007, IHC(P)
IHC(P): Rat Kidney Tissue
Click image to see more details
Anti-Calbindin-D antibody (monoclonal), MA1007, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Click image to see more details
Figure 6. Western blot analysis of Calbindin-D using anti-Calbindin-D antibody (MA1007).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates
Lane 2: rat kidney tissue lysates
Lane 3: mouse brain tissue lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Calbindin-D antigen affinity purified monoclonal antibody (Catalog # MA1007) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Calbindin-D at approximately 28KD. The expected band size for Calbindin-D is at 28KD.
Click image to see more details
Figure 7. IF analysis of Calbindin-D using anti-Calbindin-D antibody (MA1007)
Calbindin-D was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL mouse anti-Calbindin-D Antibody (MA1007) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For Calb1 (Source: Uniprot.org, NCBI)
Gene Name
Calb1
Full Name
Calbindin
Weight
29994 MW
Superfamily
calbindin family
Alternative Names
Calbindin;Calbindin D28;D-28K;Spot 35 protein;Vitamin D-dependent calcium-binding protein, avian-type;Calb1; CALB1 CALB, D-28K calbindin 1 calbindin|RTVL-H protein|calbindin 1, (28kD)|calbindin 1, 28kDa|calbindin 27|calbindin D28|vitamin D-dependent calcium-binding protein, avian-type
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Calb1, check out the Calb1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Calb1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Calbindin-D Calb1 Antibody (Monoclonal, CB-955) (MA1007)
Hello CJ!
No publications found for MA1007
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Calbindin-D Calb1 Antibody (Monoclonal, CB-955)?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Calbindin-D Calb1 Antibody (Monoclonal, CB-955)
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-Calbindin-D Calb1 Antibody (Monoclonal, CB-955)
Question
Our lab used your anti-Calbindin-D antibody (Monoclonal, CB-955) for IHC-P on kidney lung in a previous project. I am using mouse, and We want to use the antibody for IF next. I am looking for examining kidney lung as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
I looked at the website and datasheets of our anti-Calbindin-D antibody (Monoclonal, CB-955) and I see that MA1007 has been tested on mouse in both IHC-P and IF. Thus MA1007 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-04-27
Question
Would anti-Calbindin-D antibody (Monoclonal, CB-955) MA1007 work for IHC-P with brain?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
According to the expression profile of brain, CALB1 is highly expressed in brain. So, it is likely that anti-Calbindin-D antibody (Monoclonal, CB-955) MA1007 will work for IHC-P with brain.
Boster Scientific Support
Answered: 2020-04-16
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Calbindin-D antibody (Monoclonal, CB-955) MA1007. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-03
Question
We are interested in to test anti-Calbindin-D antibody (Monoclonal, CB-955) MA1007 on bovine brain for research purposes, then I may be interested in using anti-Calbindin-D antibody (Monoclonal, CB-955) MA1007 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
The products we sell, including anti-Calbindin-D antibody (Monoclonal, CB-955) MA1007, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-12
Question
Is a blocking peptide available for product anti-Calbindin-D antibody (Monoclonal, CB-955) (MA1007)?
Verified Customer
Verified customer
Asked: 2020-02-06
Answer
We do provide the blocking peptide for product anti-Calbindin-D antibody (Monoclonal, CB-955) (MA1007). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-06
Question
Is this MA1007 anti-Calbindin-D antibody (Monoclonal, CB-955) reactive to the isotypes of CALB1?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
The immunogen of MA1007 anti-Calbindin-D antibody (Monoclonal, CB-955) is Bovine kidney calbindin-D. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-20
Question
I was wanting to use your anti-Calbindin-D antibody (Monoclonal, CB-955) for IHC-P for bovine brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for bovine brain identification?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
As indicated on the product datasheet, MA1007 anti-Calbindin-D antibody (Monoclonal, CB-955) has been validated for IF, IHC-P, WB on bovine, human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in bovine brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-14
Question
Will MA1007 anti-Calbindin-D antibody (Monoclonal, CB-955) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
As indicated on the product datasheet, MA1007 anti-Calbindin-D antibody (Monoclonal, CB-955) as been validated on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-22
Question
Do you have a BSA free version of anti-Calbindin-D antibody (Monoclonal, CB-955) MA1007 available?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Calbindin-D antibody (Monoclonal, CB-955) MA1007 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-24
Question
Does anti-Calbindin-D antibody (Monoclonal, CB-955) MA1007 work on canine IF with cerebellum substantia nigra?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
Our lab technicians have not validated anti-Calbindin-D antibody (Monoclonal, CB-955) MA1007 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Calbindin-D antibody (Monoclonal, CB-955) MA1007 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine cerebellum substantia nigra in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-01
Question
My question regarding product MA1007, anti-Calbindin-D antibody (Monoclonal, CB-955). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1007 anti-Calbindin-D antibody (Monoclonal, CB-955), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-24
Question
Please see the WB image, lot number and protocol we used for brain using anti-Calbindin-D antibody (Monoclonal, CB-955) MA1007. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-04-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-27
Question
I am interested in using your anti-Calbindin-D antibody (Monoclonal, CB-955) for metanephric part of ureteric bud development studies. Has this antibody been tested with western blotting on mouse kidney tissue? We would like to see some validation images before ordering.
M. Taylor
Verified customer
Asked: 2018-03-14
Answer
We appreciate your inquiry. This MA1007 anti-Calbindin-D antibody (Monoclonal, CB-955) is tested on rat lung tissue, brain tissue, tissue lysate, kidney tissue, mouse kidney tissue, intestinal cancer tissue, mammary cancer tissue. It is guaranteed to work for IF, IHC-P, WB in bovine, human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-03-14
Question
We are currently using anti-Calbindin-D antibody (Monoclonal, CB-955) MA1007 for bovine tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says bovine, human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-22
Answer
The anti-Calbindin-D antibody (Monoclonal, CB-955) (MA1007) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-22
Question
We have seen staining in human amygdala. Do you have any suggestions? Is anti-Calbindin-D antibody (Monoclonal, CB-955) supposed to stain amygdala positively?
T. Parker
Verified customer
Asked: 2017-08-11
Answer
According to literature amygdala does express CALB1. According to Uniprot.org, CALB1 is expressed in caudate nucleus, brain, amygdala, cerebellum substantia nigra, kidney lung, among other tissues. Regarding which tissues have CALB1 expression, here are a few articles citing expression in various tissues:
Amygdala, Cerebellum, and Substantia nigra, Pubmed ID: 14702039
Brain, Pubmed ID: 2565876, 3691519
Kidney, and Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-08-11
Question
I see that the anti-Calbindin-D antibody (Monoclonal, CB-955) MA1007 works with IHC-P, what is the protocol used to produce the result images on the product page?
V. Jones
Verified customer
Asked: 2014-10-14
Answer
You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-10-14
