CALB1 Calbindin-1 Rat Recombinant Protein

Product Name

CALB1 Calbindin-1 Rat Recombinant Protein

View all Calbindin D-28K recombinant proteins

SKU/Catalog Number

PROTP07171

Size

1ug, 5ug, 100ug

Description

Recombinant Rat Calbindin-1 produced in E. coli. The Rat CALB1 is purified by proprietary chromatographic techniques. Applications: CABP28K can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection. For adsorption, we suggest the following procedure:
A- Dilute 1 µl of the monoclonal antibody against calbindin D-28k in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000).
B- Add 1 µg of the recombinant protein to 1 ml of the diluted antibody solution and mix well.
C- Incubate for at least 6 hours in the cold.
D- Apply to tissue-sections and incubate for 3 days.
E- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).
As a result, the immunostaining should be strongly reduced or even completely prevented.

Storage & Handling

Lyophilized CABP28K although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CABP28K should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Cite This Product

CALB1 Calbindin-1 Rat Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP07171)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein was lyophilized from a concentrated solution (1mg/ml) containing 50mM NaHCO₃.

Purity

Greater than 90% as determined by SDS-PAGE.

Predicted MW

30.025kDa

Reconstitution

It is recommended to reconstitute the lyophilized CABP28K in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Reconstitution

It is recommended to reconstitute the lyophilized CABP28K in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Validation Images & Assay Conditions

Gene/Protein Information For CALB1 (Source: Uniprot.org, NCBI)

Alternative Names

Calbindin; Vitamin D-dependent calcium-binding protein; avian-type; Calbindin D28; D-28K; Spot 35 protein; Calb1; CaBP28K; MGC93326 CALB1 CALB, D-28K calbindin 1 calbindin|RTVL-H protein|calbindin 1, (28kD)|calbindin 1, 28kDa|calbindin 27|calbindin D28|vitamin D-dependent calcium-binding protein, avian-type

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis