Anti-CAD/CAD Antibody Picoband™

Product Name

Anti-CAD/CAD Antibody Picoband™

View all Cad Antibodies

SKU/Catalog Number

A00463-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CAD/CAD Antibody Picoband™ catalog # A00463-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CAD/CAD Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00463-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human CAD recombinant protein (Position: L1555-K1780).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00463-1 is reactive to CAD in Human, Mouse

Applications

A00463-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

270 kDa

Calculated molecular weight

Background of Cad

The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This CAD gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of CAD using anti-CAD antibody (A00463-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse pancreas tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAD antigen affinity purified polyclonal antibody (Catalog # A00463-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAD at approximately 270KD. The expected band size for CAD is at 243KD.

Gene/Protein Information For CAD (Source: Uniprot.org, NCBI)

Alternative Names

CAD protein; CAD trifunctional protein; carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, anddihydroorotase; EC 2.1.3.2; EC 3.5.2.3; EC 6.3.5; EC 6.3.5.5; multifunctional protein CAD CAD CDG1Z, DEE50, EIEE50, GATD4 carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase CAD protein|CAD trifunctional protein|multifunctional protein CAD

For more info on CAD, check out the CAD Infographic

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In this infographic, you will see the following information for CAD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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