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Product Info Summary
| SKU: | A00463-3 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-CAD Antibody (Center)
SKU/Catalog Number
A00463-3
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-CAD Antibody (Center) (Catalog # A00463-3). Tested in WB, IHC-P, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CAD Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00463-3)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This CAD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 780-809 amino acids from the Central region of human CAD.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00463-3 is reactive to CAD in Human
Reconstitution
Calculated molecular weight
242984 Da
Background of Cad
The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00463-3 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:25
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-CAD Antibody (Center) at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: SW620 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 243 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Anti-CAD Antibody (Center) at 1:2000 dilution + 293T/17 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 243 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
All lanes : Anti-CAD Antibody (Center) at 1:2000 dilution
Lane 1: Jurkat whole cell lysate
Lane 2: 293T/17 whole cell lysate
Lane 3: Hela whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 243 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
CAD Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CAD antibody detected the CAD protein (arrow).
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CAD Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CAD Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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A00463-3 staining CAD in human placenta tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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Overlay histogram showing Hela cells stained with A00463-3 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (A00463-3, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For CAD (Source: Uniprot.org, NCBI)
Gene Name
CAD
Full Name
CAD protein
Weight
242984 Da
Alternative Names
CAD protein, Glutamine-dependent carbamoyl-phosphate synthase, Aspartate carbamoyltransferase, Dihydroorotase, CAD CAD CDG1Z, DEE50, EIEE50, GATD4 carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase CAD protein|CAD trifunctional protein|multifunctional protein CAD
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CAD, check out the CAD Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CAD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CAD Antibody (Center) (A00463-3)
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