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Product Info Summary
| SKU: | PA2308-1 |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-C5/C5a Antibody Picoband®
SKU/Catalog Number
PA2308-1
Size
100μg/vial
Form
Lyophilized
Description
Boster Bio Anti-C5/C5a Antibody catalog # PA2308-1. Tested in WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-C5/C5a Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2308-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of rat C5a, different from the related mouse sequence by five amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins
Reactive Species
PA2308-1 is reactive to C5 in Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
42 kDa
Calculated molecular weight
8981 MW
Background of C5
C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2308-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat
Positive Control
WB: Rat Liver Tissue
Validation Images & Assay Conditions
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Anti-C5/C5a antibody, PA2308-1, All Western blotting
All lanes: Anti-C5a(PA2308-1) at 0.5ug/ml
WB: Rat Liver Tissue Lysate at 40ug
Predicted bind size: 115KD
Observed bind size: 115KD
Protein Target Info & Infographic
Gene/Protein Information For C5 (Source: Uniprot.org, NCBI)
Gene Name
C5
Full Name
Complement C5
Weight
8981 MW
Alternative Names
Complement C5;C5a anaphylatoxin;C5; C5 C5Da, C5b, CPAMD4, ECLZB, C5 complement C5 complement C5|C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4|C5a anaphylatoxin|anaphylatoxin C5a analog|complement component 5|prepro-C5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on C5, check out the C5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for C5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-C5/C5a Antibody Picoband® (PA2308-1)
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No publications found for PA2308-1
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-C5/C5a Antibody Picoband®
Question
Do you have any data showing that your antibody can detect C5a, the LMW cleavage product of C5 (~14.5 kDa)?
Verified Customer
Verified customer
Asked: 2019-04-02
Answer
No, it can't recognize the LMW cleavage product of C5.
Boster Scientific Support
Answered: 2019-04-02
