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Product Info Summary
| SKU: | A00516-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA |
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Product info
Product Name
Anti-PON1 Antibody
SKU/Catalog Number
A00516-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PON1 Antibody catalog # A00516-1. Tested in ELISA applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PON1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00516-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse PON1 recombinant protein (Position: A30-D274).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00516-1 is reactive to Pon1 in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
43 kDa
Calculated molecular weight
39.731kDa
Background of PON1
Serum paraoxonase/arylesterase 1 (PON1), also known as aromatic esterase 1, is an enzyme that in humans is encoded by the PON1 gene. It is mapped to 7q21.3. This gene has esterase and more specifically paraoxonase activity. PON1 is responsible for hydrolysing organophosphate pesticides and nerve gasses. Polymorphisms in the PON1 gene significantly affect the catalytic ability of the enzyme. PON1 (paraoxonase 1) is also a major anti-atherosclerotic component of high-density lipoprotein (HDL). The PON1 gene is activated by PPAR-γ, which increases synthesis and release of paraoxonase 1 enzyme from the liver, reducing atherosclerosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00516-1 is guaranteed for ELISA Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA Capture (Matched Antibody Pair), 2-8μg/ml
Validation Images & Assay Conditions
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Figure 1. Sandwich ELISA - Recombinant mouse PON1 protein standard curve.
Use in combination with reagents from Mouse PON1 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1621).
Protein Target Info & Infographic
Gene/Protein Information For Pon1 (Source: Uniprot.org, NCBI)
Gene Name
Pon1
Full Name
Serum paraoxonase/arylesterase 1
Weight
39.731kDa
Superfamily
paraoxonase family
Alternative Names
Serum paraoxonase/arylesterase 1; PON 1; 3.1.1.2 PON1 ESA, MVCD5, PON paraoxonase 1 serum paraoxonase/arylesterase 1|A-esterase 1|K-45|PON 1|aromatic esterase 1|arylesterase 1|arylesterase B-type|esterase A|paraoxonase B-type|serum aryldiakylphosphatase|serum aryldialkylphosphatase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Pon1, check out the Pon1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Pon1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PON1 Antibody (A00516-1)
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