Anti-PON1 Antibody Picoband®

Product Name

Anti-PON1 Antibody Picoband®

View all PON1 Antibodies

SKU/Catalog Number

PA2253

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PON1 Antibody catalog # PA2253. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PON1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2253)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human PON1, different from the related mouse and rat sequences by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2253 is reactive to PON1 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

40 kDa

Calculated molecular weight

39731 MW

Background of PON1

Serum paraoxonase/arylesterase 1 (PON1), also known as aromatic esterase 1, is an enzyme that in humans is encoded by the PON1 gene. It is mapped to 7q21.3. This gene has esterase and more specifically paraoxonase activity. PON1 is responsible for hydrolysing organophosphate pesticides and nerve gasses. Polymorphisms in the PON1 gene significantly affect the catalytic ability of the enzyme. PON1 (paraoxonase 1) is also a major anti-atherosclerotic component of high-density lipoprotein (HDL). The PON1 gene is activated by PPAR-gamma, which increases synthesis and release of paraoxonase 1 enzyme from the liver, reducing atherosclerosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA2253 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Positive Control

WB: Rat Liver Tissue, Rat Lung Tissue, Human Placenta Tissue, Rat Testis Tissue, HELA Whole Cell, HEPA Whole Cell, A549 Whole Cell, JURKAT Whole Cell, SKOV Whole Cell

Validation Images & Assay Conditions

Gene/Protein Information For PON1 (Source: Uniprot.org, NCBI)

Alternative Names

Serum paraoxonase/arylesterase 1;PON 1;3.1.1.2;3.1.1.81;3.1.8.1;Aromatic esterase 1;A-esterase 1;K-45;Serum aryldialkylphosphatase 1;PON1;PON; PON1 ESA, MVCD5, PON paraoxonase 1 serum paraoxonase/arylesterase 1|A-esterase 1|K-45|PON 1|aromatic esterase 1|arylesterase 1|arylesterase B-type|esterase A|paraoxonase B-type|serum aryldiakylphosphatase|serum aryldialkylphosphatase 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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