Product Info Summary
SKU: | M05613-2 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Monkey, Mouse, Rat |
Host: | Mouse |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Beta-tubulin TUBB4A Monoclonal Antibody
View all Tubulin Beta 4 Antibodies
SKU/Catalog Number
M05613-2
Size
100ul
Description
Boster Bio Anti-Beta-tubulin TUBB4A Monoclonal Antibody catalog # M05613-2. Tested in IF, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Beta-tubulin TUBB4A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05613-2)
Host
Mouse
Contents
Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Monoclonal
Immunogen
Pig brain tubulin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05613-2 is reactive to TUBB4A in Human, Monkey, Mouse, Rat
Reconstitution
Calculated molecular weight
49586 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05613-2 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:5,000-1:10,000, ICC/IF and IHC: 1:1000-1:5,000.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of equal amounts of cell line and whole brain lysates using mouse mAb to -tubulin M05613-2, dilution 1:5,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS-1, [6] NIH-3T3, [7] C6 cells, [8] rat brain, and [9] mouse brain. Strong band at 50 kDa corresponds to the -tubulin proteins.
Click image to see more details
Immunofluorescence analysis of HeLa cells stained with mouse mAb to -tubulin, M05613-2, dilution 1:5,000 in green, and costained with chicken pAb to lamin A/C, dilution 1:2,000, in red. Blue is DAPI staining of nuclear DNA. M05613-2 antibody produces strong staining of cytoplasmic microtubules, while the lamin A/C antibody specifically labels the nuclear membrane of these cells.
Protein Target Info & Infographic
Gene/Protein Information For TUBB4A (Source: Uniprot.org, NCBI)
Gene Name
TUBB4A
Full Name
Tubulin beta-4A chain
Weight
49586 MW
Superfamily
tubulin family
Alternative Names
Tubulin beta-4A chain;Tubulin 5 beta;Tubulin beta-4 chain;TUBB4A;TUBB4, TUBB5; TUBB4A DYT4, TUBB4, beta-5 tubulin beta 4A class IVa tubulin beta-4A chain|dystonia 4, torsion (autosomal dominant)|tubulin beta-4 chain|tubulin, beta 4|tubulin, beta, 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TUBB4A, check out the TUBB4A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TUBB4A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Beta-tubulin TUBB4A Monoclonal Antibody (M05613-2)
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