Product Info Summary
SKU: | M01857-1 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-beta III Tubulin TUBB3 Rabbit Monoclonal Antibody
View all beta-III Tubulin Antibodies
SKU/Catalog Number
M01857-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-beta III Tubulin TUBB3 Rabbit Monoclonal Antibody catalog # M01857-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-beta III Tubulin TUBB3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01857-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CC-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human beta III Tubulin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01857-1 is reactive to TUBB3 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
50 kDa
Calculated molecular weight
50433 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01857-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: human SH-SY5Y whole cell, human SIHA whole cell, rat brain tissue, mouse brain tissue
IHC: human testicular seminoma tissue, human testicular seminoma tissue, human thyroid papillary carcinoma tissue, human thyroid papillary carcinoma tissue, human tonsil tissue, human tonsil tissue, human urothelial carcinoma tissue, human urothelial carcinoma tissue, non-small cell lung cancer tissue, non-small cell lung cancer tissue
ICC/IF: A431 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TUBB3 using anti-TUBB3 antibody (M01857-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human SIHA whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBB3 antigen affinity purified polyclonal antibody (Catalog # M01857-1) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TUBB3 at approximately 50 kDa. The expected band size for TUBB3 is at 50 kDa.
Click image to see more details
Figure 2. IHC analysis of TUBB3 using anti-TUBB3 antibody (M01857-1).
TUBB3 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TUBB3 Antibody (M01857-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of TUBB3 using anti-TUBB3 antibody (M01857-1).
TUBB3 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TUBB3 Antibody (M01857-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of TUBB3 using anti-TUBB3 antibody (M01857-1).
TUBB3 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TUBB3 Antibody (M01857-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of TUBB3 using anti-TUBB3 antibody (M01857-1).
TUBB3 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TUBB3 Antibody (M01857-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of TUBB3 using anti-TUBB3 antibody (M01857-1).
TUBB3 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TUBB3 Antibody (M01857-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 7. IHC analysis of TUBB3 using anti-TUBB3 antibody (M01857-1).
TUBB3 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TUBB3 Antibody (M01857-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 8. IHC analysis of TUBB3 using anti-TUBB3 antibody (M01857-1).
TUBB3 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TUBB3 Antibody (M01857-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 9. IHC analysis of TUBB3 using anti-TUBB3 antibody (M01857-1).
TUBB3 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TUBB3 Antibody (M01857-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 10. IHC analysis of TUBB3 using anti-TUBB3 antibody (M01857-1).
TUBB3 was detected in a paraffin-embedded section of non-small cell lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TUBB3 Antibody (M01857-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 11. IHC analysis of TUBB3 using anti-TUBB3 antibody (M01857-1).
TUBB3 was detected in a paraffin-embedded section of non-small cell lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TUBB3 Antibody (M01857-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Immunofluorescent analysis of A431 cells, using beta III Tubulin Antibody .
Protein Target Info & Infographic
Gene/Protein Information For TUBB3 (Source: Uniprot.org, NCBI)
Gene Name
TUBB3
Full Name
Tubulin beta-3 chain
Weight
50433 MW
Superfamily
tubulin family
Alternative Names
Tubulin beta-3 chain;Tubulin beta-4 chain;Tubulin beta-III;TUBB3;TUBB4; TUBB3 CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4 tubulin beta 3 class III tubulin beta-3 chain|class III beta-tubulin|tubulin beta-4 chain|tubulin beta-III|tubulin, beta 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TUBB3, check out the TUBB3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TUBB3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-beta III Tubulin TUBB3 Rabbit Monoclonal Antibody (M01857-1)
Hello CJ!
M01857-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Karami S,Doroodmand MM,Taherianfar M,Mutabi-Alavi A,Nagshgar N.Mechanism behind the neuronal ephaptic coupling during synchronizing by specific brain-triggered wave as neuronal motor toolkit.Sci Rep.2021 Feb 11;11(1):3683.doi:10.1038/s41598-021-82118-2.PM
Species: Rat,Snail
Karami S,Doroodmand MM,Taherianfar M,Mutabi-Alavi A,Nagshgar N.Mechanism behind the neuronal ephaptic coupling during synchronizing by specific brain-triggered wave as neuronal motor toolkit.Sci Rep.2021 Feb 11;11(1):3683.doi:10.1038/s41598-021-82118-2.PM
Species: Rat,Snail
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Customer Q&As
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4 Customer Q&As for Anti-beta III Tubulin TUBB3 Rabbit Monoclonal Antibody
Question
We are currently using anti-beta III Tubulin Rabbit Monoclonal antibody M01857-1 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
The anti-beta III Tubulin Rabbit Monoclonal antibody (M01857-1) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-06
Question
I was wanting to use your anti-beta III Tubulin Rabbit Monoclonal antibody for WB for mouse hypothalamus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse hypothalamus identification?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
As indicated on the product datasheet, M01857-1 anti-beta III Tubulin Rabbit Monoclonal antibody has been validated for IP, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse hypothalamus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-28
Question
Here is the WB image, lot number and protocol we used for hypothalamus using anti-beta III Tubulin Rabbit Monoclonal antibody M01857-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-21
Question
Does anti-beta III Tubulin Rabbit Monoclonal antibody M01857-1 work for WB with hypothalamus?
Verified Customer
Verified customer
Asked: 2017-08-23
Answer
According to the expression profile of hypothalamus, TUBB3 is highly expressed in hypothalamus. So, it is likely that anti-beta III Tubulin Rabbit Monoclonal antibody M01857-1 will work for WB with hypothalamus.
Boster Scientific Support
Answered: 2017-08-23