Anti-Phospho-beta-catenin (Ser33/37) Antibody

Product Name

Anti-Phospho-beta-catenin (Ser33/37) Antibody

View all beta-Catenin Antibodies

SKU/Catalog Number

M00004-4

Size

50 µl

Description

Boster Bio Anti-Phospho-beta-catenin (Ser33/37) Antibody (Catalog # M00004-4). Tested in WB application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-Phospho-beta-catenin (Ser33/37) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00004-4)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This Phospho-beta-catenin (Ser33/37) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 19-41 amino acids from human beta-catenin .

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00004-4 is reactive to CTNNB1 in Human

Reconstitution

Calculated molecular weight

85497 Da

Background of beta-Catenin

Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22647378, PubMed:22699938, PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00004-4 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

Gene/Protein Information For CTNNB1 (Source: Uniprot.org, NCBI)

Alternative Names

Catenin beta-1, Beta-catenin, CTNNB1, CTNNB CTNNB1 CTNNB, EVR7, MRD19, NEDSDV, armadillo catenin beta 1 catenin beta-1|catenin (cadherin-associated protein), beta 1, 88kDa

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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