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Product Info Summary
| SKU: | PA1014 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-BDNF Antibody Picoband®
SKU/Catalog Number
PA1014
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-BDNF Antibody catalog # PA1014. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BDNF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1014)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminal of human BDNF, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1014 is reactive to BDNF in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
37 kDa
Calculated molecular weight
27818 MW
Background of Bdnf
Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes NGF, NT3, and NT4. BDNF is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields. BDNF has been purified and shown to reduce the amount of naturally occurring neuronal cell death in portions of the peripheral nervous system.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1014 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Brain Tissue, Mouse Brain Tissue, MCF-7 Whole Cell
Validation Images & Assay Conditions
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Anti-BDNF antibody, PA1014, Western blotting
All lanes: Anti BDNF (PA1014) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 28KD
Observed bind size: 37KD
Protein Target Info & Infographic
Gene/Protein Information For BDNF (Source: Uniprot.org, NCBI)
Gene Name
BDNF
Full Name
Brain-derived neurotrophic factor
Weight
27818 MW
Superfamily
NGF-beta family
Alternative Names
Brain-derived neurotrophic factor;BDNF;Abrineurin;BDNF; BDNF ANON2, BULN2 brain derived neurotrophic factor brain-derived neurotrophic factor|abrineurin|neurotrophin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BDNF, check out the BDNF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BDNF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BDNF Antibody Picoband® (PA1014)
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Customer Q&As
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9 Customer Q&As for Anti-BDNF Antibody Picoband®
Question
Does PA1014 react to mature, precursor, or both forms of BDNF? Keywords: pro-BDNF, m-BDNF, isoform
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
PA1014 reacts to pre-cursor BDNF.
Boster Scientific Support
Answered: 2020-04-30
Question
For the BDNF antibody associated with ECL detection, what type of gel and gradient do you use?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
We use SDS-PAGE gel with 5% concentrated gel and 12% resolving gel.
Boster Scientific Support
Answered: 2020-04-16
Question
Will anti-BDNF antibody PA1014 work on primate WB with leukocyte?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
Our lab technicians have not tested anti-BDNF antibody PA1014 on primate. You can run a BLAST between primate and the immunogen sequence of anti-BDNF antibody PA1014 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate leukocyte in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-07
Question
Please see the WB image, lot number and protocol we used for serum using anti-BDNF antibody PA1014. Please let me know if you require anything else.
J. Jha
Verified customer
Asked: 2018-06-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-15
Question
My question regards to test anti-BDNF antibody PA1014 on rat serum for research purposes, then I may be interested in using anti-BDNF antibody PA1014 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-02-26
Answer
The products we sell, including anti-BDNF antibody PA1014, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-02-26
Question
I was wanting to use your anti-BDNF antibody for WB for rat serum on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat serum identification?
K. Jackson
Verified customer
Asked: 2016-11-10
Answer
As indicated on the product datasheet, PA1014 anti-BDNF antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat serum in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-11-10
Question
Is there a BSA free version of anti-BDNF antibody PA1014 available?
P. Jha
Verified customer
Asked: 2016-01-26
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-BDNF antibody PA1014 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-01-26
Question
Would PA1014 anti-BDNF antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Z. Jackson
Verified customer
Asked: 2015-10-22
Answer
It shows on the product datasheet, PA1014 anti-BDNF antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-10-22
Question
Is a blocking peptide available for product anti-BDNF antibody (PA1014)?
J. Kulkarni
Verified customer
Asked: 2013-12-31
Answer
We do provide the blocking peptide for product anti-BDNF antibody (PA1014). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-12-31
