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Product Info Summary
| SKU: | M01616-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Bcl10 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01616-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Bcl10 Rabbit Monoclonal Antibody catalog # M01616-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bcl10 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01616-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GDB-2
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Bcl10
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01616-1 is reactive to BCL10 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
65 kDa
Calculated molecular weight
26252 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01616-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:20
Positive Control
WB: HeLa cell, Raji cell
IHC: human breast cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of Bcl10 expression in (1) HeLa cell lysate; (2) Raji cell lysate.
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Immunohistochemical analysis of paraffin-embedded human breast cancer, using Bcl10 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For BCL10 (Source: Uniprot.org, NCBI)
Gene Name
BCL10
Full Name
B-cell lymphoma/leukemia 10
Weight
26252 MW
Alternative Names
B-cell lymphoma/leukemia 10;B-cell CLL/lymphoma 10;Bcl-10;CARD-containing molecule enhancing NF-kappa-B;CARD-like apoptotic protein;hCLAP;CED-3/ICH-1 prodomain homologous E10-like regulator;CIPER;Cellular homolog of vCARMEN;cCARMEN;Cellular-E10;c-E10;Mammalian CARD-containing adapter molecule E10;mE10;BCL10;CIPER, CLAP; BCL10 CARMEN, CIPER, CLAP, IMD37, c-E10, mE10 BCL10 immune signaling adaptor B-cell lymphoma/leukemia 10|B cell CLL/lymphoma 10|CARD containing molecule enhancing NF-kB|CARD-containing apoptotic signaling protein|CARD-containing molecule enhancing NF-kappa-B|CARD-containing proapoptotic protein|CED-3/ICH-1 prodomain homologous E10-like regulator|cCARMEN|caspase-recruiting domain-containing protein|cellular homolog of vCARMEN|cellular-E10|hCLAP|mammalian CARD-containing adapter molecule E10
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BCL10, check out the BCL10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BCL10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bcl10 Rabbit Monoclonal Antibody (M01616-1)
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Customer Q&As
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4 Customer Q&As for Anti-Bcl10 Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-Bcl10 Rabbit Monoclonal antibody for WB for human epithelial cell of pancreas on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human epithelial cell of pancreas identification?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
You can see on the product datasheet, M01616-1 anti-Bcl10 Rabbit Monoclonal antibody has been tested for IP, IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human epithelial cell of pancreas in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-23
Question
Will anti-Bcl10 Rabbit Monoclonal antibody M01616-1 work for WB with epithelial cell of pancreas?
Verified Customer
Verified customer
Asked: 2018-06-08
Answer
According to the expression profile of epithelial cell of pancreas, BCL10 is highly expressed in epithelial cell of pancreas. So, it is likely that anti-Bcl10 Rabbit Monoclonal antibody M01616-1 will work for WB with epithelial cell of pancreas.
Boster Scientific Support
Answered: 2018-06-08
Question
Will M01616-1 anti-Bcl10 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-01-19
Answer
It shows on the product datasheet, M01616-1 anti-Bcl10 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-01-19
Question
Is a blocking peptide available for product anti-Bcl10 Rabbit Monoclonal antibody (M01616-1)?
C. Li
Verified customer
Asked: 2013-05-20
Answer
We do provide the blocking peptide for product anti-Bcl10 Rabbit Monoclonal antibody (M01616-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-05-20
