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Product Info Summary
| SKU: | A00101 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-IL-1 beta/IL1B Antibody Picoband®
View all IL-1 beta/IL-1F2 Antibodies
SKU/Catalog Number
A00101
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL-1 beta/IL1B Antibody Picoband® catalog # A00101. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL-1 beta/IL1B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00101)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human IL-1 beta recombinant protein (Position: A117-S269). Human IL-1 beta shares 78.3% and 77.6% amino acid (aa) sequence identity with mouse and rat IL-1 beta, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00101 is reactive to IL1B in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
35 kDa
Calculated molecular weight
30748 MW
Background of IL-1 beta/IL-1F2
FGFR1, Fibroblast growth factor receptor 1, also known as basic fibroblast growth factor receptor 1, fms-related tyrosine kinase-2 / Pfeiffer syndrome, and CD331, is a receptor tyrosine kinase whose ligands are specific members of the fibroblast growth factor family. The FGFR1 gene is localized to 8p12-p11.2 by in situ hybridization. FGFR1 is essential for the normal formation of the organ of Corti and that phenotype severity observed in FGFR1 mutants is dependent on the dose of FGFR1. Mutations in this gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer and autosomal dominant Kallmann syndrome 2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00101 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human COLO-320 whole cell, rat spleen tissue, mouse NIH3T3 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of IL-1 beta using anti-IL-1 beta antibody (A00101).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human COLO-320 whole cell lysate,
Lane 2: rat spleen tissue lysate,
Lane 3: mouse NIH3T3 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-1 beta antigen affinity purified polyclonal antibody (Catalog # A00101) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-1 beta at approximately 35KD. The expected band size for IL-1 beta is at 31KD.
Protein Target Info & Infographic
Gene/Protein Information For IL1B (Source: Uniprot.org, NCBI)
Gene Name
IL1B
Full Name
Interleukin-1 beta
Weight
30748 MW
Superfamily
IL-1 family
Alternative Names
Interleukin-1 beta;IL-1 beta;Catabolin;IL1B;IL1F2; IL1B IL-1, IL1-BETA, IL1F2, IL1beta interleukin 1 beta interleukin-1 beta|IL-1 beta|catabolin|interleukin 1beta|preinterleukin 1 beta|pro-interleukin-1-beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL1B, check out the IL1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL-1 beta/IL1B Antibody Picoband® (A00101)
Hello CJ!
A00101 has been cited in 27 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBPα
The interrupted cross-talk of inflammatory and oxidative stress trajectories signifies the effect of artesunate against hepatic ischemia/reperfusion-induced inflammasomopathy
Neuregulin-1 protects mouse cerebellum against oxidative stress and neuroinflammation
Protective effects of tannic acid on acute doxorubicin-induced cardiotoxicity: Involvement of suppression in oxidative stress, inflammation, and apoptosis
IL-17 Stimulates Migration of Carotid Artery Vascular Smooth Muscle Cells in an MMP-9 Dependent Manner via p38 MAPK and ERK1/2-Dependent NF-κB and AP-1 Activation
Adolescent cocaine exposure induces prolonged synaptic modifications in medial prefrontal cortex of adult rats
Resveratrol fails to affect cocaine conditioned place preference behavior, but alleviates anxiety-like behaviors in cocaine withdrawn rats
Shikonin protects against lipopolysaccharide-induced inflammation and apoptosis in human nucleus pulposus cells through the nuclear factor-kappa B pathway
Effects of Ultrashort Wave Therapy on Inflammation and Macrophage Polarization after Acute Lung Injury in Rats
Effects of Ultrashort Wave Therapy on Inflammation and Macrophage Polarization after Acute Lung Injury in Rats
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-IL-1 beta/IL1B Antibody Picoband®
Question
Will A00101 anti-IL-1 beta/IL1B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
You can see on the product datasheet, A00101 anti-IL-1 beta/IL1B antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-28
Question
Is this A00101 anti-IL-1 beta/IL1B antibody reactive to the isotypes of IL1B?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
The immunogen of A00101 anti-IL-1 beta/IL1B antibody is E. coli-derived human IL-1 beta recombinant protein (Position: A117-S269). Human IL-1 beta shares 78.3% and 77.6% amino acid (aa) sequence identity with mouse and rat IL-1 beta, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-14
Question
We are currently using anti-IL-1 beta/IL1B antibody A00101 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
The anti-IL-1 beta/IL1B antibody (A00101) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-30
Question
I would like to test anti-IL-1 beta/IL1B antibody A00101 on rat leukocyte for research purposes, then I may be interested in using anti-IL-1 beta/IL1B antibody A00101 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
The products we sell, including anti-IL-1 beta/IL1B antibody A00101, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-04
Question
We have seen staining in rat skin. Do you have any suggestions? Is anti-IL-1 beta/IL1B antibody supposed to stain skin positively?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
According to literature skin does express IL1B. According to Uniprot.org, IL1B is expressed in smooth muscle tissue, leukocyte, histiocytic lymphoma, monocyte, lung, skin, macrophage, among other tissues. Regarding which tissues have IL1B expression, here are a few articles citing expression in various tissues:
Histiocytic lymphoma, Pubmed ID: 3493774
Leukocyte, Pubmed ID: 3490654
Lung, Pubmed ID: 15489334
Macrophage, Pubmed ID: 20148899
Monocyte, Pubmed ID: 2635664, 11991722
Skin, Pubmed ID: 1919436
Boster Scientific Support
Answered: 2019-08-23
Question
Is a blocking peptide available for product anti-IL-1 beta/IL1B antibody (A00101)?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
We do provide the blocking peptide for product anti-IL-1 beta/IL1B antibody (A00101). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-20
Question
Do you have a BSA free version of anti-IL-1 beta/IL1B antibody A00101 available?
G. Krishna
Verified customer
Asked: 2019-06-19
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL-1 beta/IL1B antibody A00101 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-19
Question
I see that the anti-IL-1 beta/IL1B antibody A00101 works with WB, what is the protocol used to produce the result images on the product page?
C. Patel
Verified customer
Asked: 2019-05-29
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-29
Question
Do you know if A00101 recognizes the native or denatured target?
Verified customer
Asked: 2019-05-06
Answer
Yes, the Anti-IL-1 Beta/IL1B Antibody Picoband™ (A00101) recognizes the denatured target.
Boster Scientific Support
Answered: 2019-05-07
Question
Please see the WB image, lot number and protocol we used for leukocyte using anti-IL-1 beta/IL1B antibody A00101. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-04-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-16
Question
Checking back with a new antibody questions - wondering if the one I recently found below has been characterized to determine if it recognizes native or denatured target? https://www.bosterbio.com/anti-il-1-beta-picoband-trade-antibody-a00101-boster.html?options=cart#gene-info Thanks! Janice
Verified Customer
Verified customer
Asked: 2019-03-29
Answer
This antibody recognizes the denatured target
Boster Scientific Support
Answered: 2019-03-29
Question
I was wanting to use your anti-IL-1 beta/IL1B antibody for WB for rat leukocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat leukocyte identification?
Verified Customer
Verified customer
Asked: 2018-08-09
Answer
As indicated on the product datasheet, A00101 anti-IL-1 beta/IL1B antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-09
Question
Would anti-IL-1 beta/IL1B antibody A00101 work for WB with leukocyte?
Verified Customer
Verified customer
Asked: 2017-11-21
Answer
According to the expression profile of leukocyte, IL1B is highly expressed in leukocyte. So, it is likely that anti-IL-1 beta/IL1B antibody A00101 will work for WB with leukocyte.
Boster Scientific Support
Answered: 2017-11-21
Question
My question regarding product A00101, anti-IL-1 beta/IL1B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-10-26
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00101 anti-IL-1 beta/IL1B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-26
Question
I was wanting to use using your anti-IL-1 beta/IL1B antibody for response to lipopolysaccharide studies. Has this antibody been tested with western blotting on rat spleen tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-07-04
Answer
Thank you for your inquiry. This A00101 anti-IL-1 beta/IL1B antibody is validated on rat spleen tissue, tissue lysate, mouse nih3t3 whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-07-04
Question
Our lab were content with the WB result of your anti-IL-1 beta/IL1B antibody. However we have observed positive staining in macrophage cytoplasm using this antibody. Is that expected? Could you tell me where is IL1B supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-05-31
Answer
According to literature, macrophage does express IL1B. Generally IL1B expresses in cytoplasm, cytosol. Regarding which tissues have IL1B expression, here are a few articles citing expression in various tissues:
Histiocytic lymphoma, Pubmed ID: 3493774
Leukocyte, Pubmed ID: 3490654
Lung, Pubmed ID: 15489334
Macrophage, Pubmed ID: 20148899
Monocyte, Pubmed ID: 2635664, 11991722
Skin, Pubmed ID: 1919436
Boster Scientific Support
Answered: 2017-05-31
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-IL-1 beta/IL1B antibody A00101. Let me know if you need anything else.
K. Yang
Verified customer
Asked: 2016-10-20
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-10-20
