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Product Info Summary
| SKU: | A03520S136 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Bad (phospho-S136) Antibody
SKU/Catalog Number
A03520S136
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Bad (phospho-S136) Antibody catalog # A03520S136. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bad (phospho-S136) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03520S136)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phosphopeptide derived from human Bad around the phosphorylation site of Serine 136.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03520S136 is reactive to BAD in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
18392 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03520S136 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of BAD using Anti-Bad (phospho-S136) Antibody (A03520S136).
Immunohistochemistry (IHC) analyzes of p-Bad (S136) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-Bad (phospho-S136) Antibody A03520S136
Western blot (WB) analysis of Bad (phospho-S136) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate(40ug)
Lane2:HEK293T treated with UV for 1h whole cell lysate(40ug)
Lane3:HEK293T treated with UV for 4h whole cell lysate(40ug)
Lane4:HEK293T treated with UV for 16h whole cell lysate(40ug)
Lane5:HEK293T treated with UV for 24h whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For BAD (Source: Uniprot.org, NCBI)
Gene Name
BAD
Full Name
Bcl2-associated agonist of cell death
Weight
18392 MW
Superfamily
Bcl-2 family
Alternative Names
Bcl2-associated agonist of cell death;BAD;Bcl-2-binding component 6;Bcl-2-like protein 8;Bcl2-L-8;Bcl-xL/Bcl-2-associated death promoter;Bcl2 antagonist of cell death;BAD;BBC6, BCL2L8; BAD BBC2, BCL2L8 BCL2 associated agonist of cell death bcl2-associated agonist of cell death|BCL-X/BCL-2 binding protein|BCL2-antagonist of cell death protein|BCL2-binding component 6|BCL2-binding protein|bcl-2-binding component 6|bcl-2-like protein 8|bcl-XL/Bcl-2-associated death promoter|bcl2 antagonist of cell death|bcl2-L-8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BAD, check out the BAD Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BAD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bad (phospho-S136) Antibody (A03520S136)
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