Anti-Parkin/PRKN Antibody Picoband®

Parkin antibody

Boster Bio Anti-Parkin/PRKN Antibody Picoband® catalog # PB9307. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PB9307
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, ICC, WB

Product Name

Anti-Parkin/PRKN Antibody Picoband®

View all Parkin Antibodies

SKU/Catalog Number

PB9307

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Parkin/PRKN Antibody Picoband® catalog # PB9307. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Parkin/PRKN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9307)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Parkin recombinant protein (Position: I23-K416). Human Parkin shares 82% and 84% amino acid (aa) sequence identity with mouse and rat Parkin, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9307 is reactive to PRKN in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

66 kDa

Calculated molecular weight

51641 MW

Background of Parkin

Parkin is a RING domain-containing E3 ubiquitin ligase involved in proteasome-dependent degradation of proteins. It is mapped to 6q26. This gene is important for mitochondrial quality control by lysosome-dependent degradation of damaged mitochondria through autophagy, or mitophagy. Parkin is expressed in neuronal processes and cell bodies of neurons, but not glial cells, in the midbrain, basal ganglia, cerebral cortex, and cerebellum. Parkin assimilated with actin filaments, suggesting that it is a cytoskeletal-associated protein. Parkin is identified as a transcriptional repressor of p53 independent of its ubiquitin ligase function. It also has been found that parkin was associated physically with mitochondrial DNA (mtDNA) in proliferating as well as in differentiated SH-SY5Y neuroblastoma cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9307 is guaranteed for IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunocytochemistry , 0.5-1μg/ml, Mouse, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Positive Control

WB: U87 Whole Cell, Mouse Brain Tissue
IHC: Mouse Brain Tissue, Rat Brain Tissue, Human Intestinal Cancer Tissue
ICC: NEURO-2α cell

Validation Images & Assay Conditions

Gene/Protein Information For PRKN (Source: Uniprot.org, NCBI)

Gene Name

PRKN

Full Name

E3 ubiquitin-protein ligase parkin

Weight

51641 MW

Superfamily

RBR family

Alternative Names

E3 ubiquitin-protein ligase parkin;Parkin;6.3.2.-;Parkinson juvenile disease protein 2;Parkinson disease protein 2;PARK2;PRKN; PRKN AR-JP, LPRS2, PARK2, PDJ parkin RBR E3 ubiquitin protein ligase E3 ubiquitin-protein ligase parkin|Parkinson disease (autosomal recessive, juvenile) 2, parkin|parkinson juvenile disease protein 2|parkinson protein 2 E3 ubiquitin protein ligase|parkinson protein 2, E3 ubiquitin protein ligase (parkin)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PRKN, check out the PRKN Infographic

PRKN infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PRKN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-Parkin/PRKN Antibody Picoband®

Question

Can you help my question with product PB9307, anti-Parkin/PRKN antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9307 anti-Parkin/PRKN antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-01-08

Question

We are currently using anti-Parkin/PRKN antibody PB9307 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-01

Answer

The anti-Parkin/PRKN antibody (PB9307) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-01

Question

Is a blocking peptide available for product anti-Parkin/PRKN antibody (PB9307)?

M. Zhao

Verified customer

Asked: 2018-09-21

Answer

We do provide the blocking peptide for product anti-Parkin/PRKN antibody (PB9307). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-09-21

Question

Is this PB9307 anti-Parkin/PRKN antibody reactive to the isotypes of PARK2?

Verified Customer

Verified customer

Asked: 2017-06-07

Answer

The immunogen of PB9307 anti-Parkin/PRKN antibody is E.coli-derived human Parkin recombinant protein (Position: I23-K416). Human Parkin shares 82% and 84% amino acid (aa) sequence identity with mouse and rat Parkin, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-06-07

Order DetailsPrice
PB9307

100μg

$370
PB9307-10ug

10μg sample (liquid)

$99
PB9307-Biotin

100 μg Biotin conjugated

$570
PB9307-Cy3

100 μg Cy3 conjugated

$570
PB9307-Dylight488

100 μg Dylight488 conjugated

$570
PB9307-Dylight550

100 μg Dylight550 conjugated

$570
PB9307-Dylight594

100 μg Dylight594 conjugated

$570
PB9307-FITC

100 μg FITC conjugated

$570
PB9307-HRP

100 μg HRP conjugated

$570
PB9307-APC

100 μg APC conjugated

$670
PB9307-PE

100 μg PE conjugated

$670
PB9307-iFluor647

100 μg iFluor647 conjugated

$670
PB9307-carrier-free

Carrier Free

$370

More conjugates/formats

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PB9307
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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