Product Info Summary
SKU: | PB9307 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-Parkin/PRKN Antibody Picoband®
SKU/Catalog Number
PB9307
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Parkin/PRKN Antibody Picoband® catalog # PB9307. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Parkin/PRKN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9307)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Parkin recombinant protein (Position: I23-K416). Human Parkin shares 82% and 84% amino acid (aa) sequence identity with mouse and rat Parkin, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9307 is reactive to PRKN in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
66 kDa
Calculated molecular weight
51641 MW
Background of Parkin
Parkin is a RING domain-containing E3 ubiquitin ligase involved in proteasome-dependent degradation of proteins. It is mapped to 6q26. This gene is important for mitochondrial quality control by lysosome-dependent degradation of damaged mitochondria through autophagy, or mitophagy. Parkin is expressed in neuronal processes and cell bodies of neurons, but not glial cells, in the midbrain, basal ganglia, cerebral cortex, and cerebellum. Parkin assimilated with actin filaments, suggesting that it is a cytoskeletal-associated protein. Parkin is identified as a transcriptional repressor of p53 independent of its ubiquitin ligase function. It also has been found that parkin was associated physically with mitochondrial DNA (mtDNA) in proliferating as well as in differentiated SH-SY5Y neuroblastoma cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9307 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry , 0.5-1μg/ml, Mouse, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: U87 Whole Cell, Mouse Brain Tissue
IHC: Mouse Brain Tissue, Rat Brain Tissue, Human Intestinal Cancer Tissue
ICC: NEURO-2α cell
Validation Images & Assay Conditions
Click image to see more details
Anti-Parkin Picoband antibody, PB9307, Western blotting
All lanes: Anti Parkin (PB9307) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 66KD
Observed bind size: 66KD
Click image to see more details
Anti-Parkin Picoband antibody, PB9307, IHC(P)
IHC(P): Mouse Brain Tissue
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Anti-Parkin Picoband antibody, PB9307, IHC(P)
IHC(P): Rat Brain Tissue
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Anti-Parkin Picoband antibody, PB9307, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
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Figure 5. IHC analysis of Parkin using anti-Parkin antibody (PB9307).
Parkin was detected in immunocytochemical section of NEURO-2α Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-Parkin Antibody (PB9307) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For PRKN (Source: Uniprot.org, NCBI)
Gene Name
PRKN
Full Name
E3 ubiquitin-protein ligase parkin
Weight
51641 MW
Superfamily
RBR family
Alternative Names
E3 ubiquitin-protein ligase parkin;Parkin;6.3.2.-;Parkinson juvenile disease protein 2;Parkinson disease protein 2;PARK2;PRKN; PRKN AR-JP, LPRS2, PARK2, PDJ parkin RBR E3 ubiquitin protein ligase E3 ubiquitin-protein ligase parkin|Parkinson disease (autosomal recessive, juvenile) 2, parkin|parkinson juvenile disease protein 2|parkinson protein 2 E3 ubiquitin protein ligase|parkinson protein 2, E3 ubiquitin protein ligase (parkin)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKN, check out the PRKN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Parkin/PRKN Antibody Picoband® (PB9307)
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Customer Q&As
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4 Customer Q&As for Anti-Parkin/PRKN Antibody Picoband®
Question
Can you help my question with product PB9307, anti-Parkin/PRKN antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9307 anti-Parkin/PRKN antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-08
Question
We are currently using anti-Parkin/PRKN antibody PB9307 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-01
Answer
The anti-Parkin/PRKN antibody (PB9307) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-01
Question
Is a blocking peptide available for product anti-Parkin/PRKN antibody (PB9307)?
M. Zhao
Verified customer
Asked: 2018-09-21
Answer
We do provide the blocking peptide for product anti-Parkin/PRKN antibody (PB9307). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-09-21
Question
Is this PB9307 anti-Parkin/PRKN antibody reactive to the isotypes of PARK2?
Verified Customer
Verified customer
Asked: 2017-06-07
Answer
The immunogen of PB9307 anti-Parkin/PRKN antibody is E.coli-derived human Parkin recombinant protein (Position: I23-K416). Human Parkin shares 82% and 84% amino acid (aa) sequence identity with mouse and rat Parkin, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-06-07