Anti-Phospho-Bad (S112) Monoclonal Antibody

BAD antibody

Boster Bio Anti-Phospho-Bad (S112) Monoclonal Antibody catalog # MP03520. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: MP03520
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IP, WB

Product Name

Anti-Phospho-Bad (S112) Monoclonal Antibody

View all BAD Antibodies

SKU/Catalog Number

MP03520

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Phospho-Bad (S112) Monoclonal Antibody catalog # MP03520. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-Bad (S112) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MP03520)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AEBH-2

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Bad Promotes cell death. Successfully competes for the binding to Bcl-X (L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

MP03520 is reactive to BAD in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

15 kDa

Calculated molecular weight

26053 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

MP03520 is guaranteed for IP, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IP 1:20

Positive Control

WB: HeLa cell, HeLa cell

Validation Images & Assay Conditions

Gene/Protein Information For BAD (Source: Uniprot.org, NCBI)

Gene Name

BAD

Full Name

Bcl2-associated agonist of cell death

Weight

26053 MW

Superfamily

Bcl-2 family

Alternative Names

Red fluorescent protein eqFP611;GFP-like chromoprotein; BAD BBC2, BCL2L8 BCL2 associated agonist of cell death bcl2-associated agonist of cell death|BCL-X/BCL-2 binding protein|BCL2-antagonist of cell death protein|BCL2-binding component 6|BCL2-binding protein|bcl-2-binding component 6|bcl-2-like protein 8|bcl-XL/Bcl-2-associated death promoter|bcl2 antagonist of cell death|bcl2-L-8

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on BAD, check out the BAD Infographic

BAD infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for BAD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

MP03520 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wang Q, Xu Y, Zhou W, Zhong L, Wen Z, Yu H, Chen S, Shen J, Chen H, She Q, Jiang J, Miao J, Wei W. Int J Biol Sci. 2014 Nov 18;10(10):1181-92. Doi: 10.7150/Ijbs.10275. Ecollection 2014. The Viral Oncoprotein Hbx Of Hepatitis B Virus Promotes The G...

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5 Customer Q&As for Anti-Phospho-Bad (S112) Monoclonal Antibody

Question

My question regarding product MP03520, anti-Phospho-Bad (S112) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-01-29

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MP03520 anti-Phospho-Bad (S112) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-01-29

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Phospho-Bad (S112) Monoclonal antibody MP03520. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-06-10

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-10

Question

We are currently using anti-Phospho-Bad (S112) Monoclonal antibody MP03520 for mouse tissue, and we are happy with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-07

Answer

The anti-Phospho-Bad (S112) Monoclonal antibody (MP03520) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-07

Question

Will anti-Phospho-Bad (S112) Monoclonal antibody MP03520 work for WB with cervix carcinoma erythroleukemia?

Verified Customer

Verified customer

Asked: 2017-07-21

Answer

According to the expression profile of cervix carcinoma erythroleukemia, BAD is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-Phospho-Bad (S112) Monoclonal antibody MP03520 will work for WB with cervix carcinoma erythroleukemia.

Boster Scientific Support

Answered: 2017-07-21

Question

Our lab want to know about to test anti-Phospho-Bad (S112) Monoclonal antibody MP03520 on mouse cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-Phospho-Bad (S112) Monoclonal antibody MP03520 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

C. Edwards

Verified customer

Asked: 2013-08-26

Answer

The products we sell, including anti-Phospho-Bad (S112) Monoclonal antibody MP03520, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-08-26

Order DetailsPrice
MP03520

100μL

$370
MP03520-30ul

30μL sample

$99
MP03520-carrier-free-1ml

Carrier Free

$2590

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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