Product Info Summary
SKU: | M00762-1 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Aurora B AURKB Rabbit Monoclonal Antibody
SKU/Catalog Number
M00762-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Aurora B AURKB Rabbit Monoclonal Antibody catalog # M00762-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Aurora B AURKB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00762-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FA-1
Isotype
Rabbit IgG
Standard Protein
A synthesized peptide derived from human Aurora B
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00762-1 is reactive to AURKB in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
39 kDa
Calculated molecular weight
39311 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00762-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: human Hela whole cell, human 293T whole cell, human Jurkat whole cell, human U251 whole cell
IHC: human bladder urothelial carcinoma tissue, human tonsil tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Aurora B using anti-Aurora B antibody (M00762-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human U251 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aurora B antigen affinity purified monoclonal antibody (Catalog # M00762-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aurora B at approximately 39 kDa. The expected band size for Aurora B is at 39 kDa.
Click image to see more details
Figure 2. IHC analysis of AURKB using anti-AURKB antibody (M00762-1).
AURKB was detected in a paraffin-embedded section of human bladder urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-AURKB Antibody (M00762-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of AURKB using anti-AURKB antibody (M00762-1).
AURKB was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-AURKB Antibody (M00762-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For AURKB (Source: Uniprot.org, NCBI)
Gene Name
AURKB
Full Name
Aurora kinase B
Weight
39311 MW
Superfamily
protein kinase superfamily
Alternative Names
Aik2; AIK2EC 2.7.11.1; AIM1; AIM-1; AIM1Aik2; AIM-1STK-1; ARK2; ARK2ARK-2; AurB; AURKB; aurkb-sv2; Aurora- and IPL1-like midbody-associated protein 1; Aurora B; aurora kinase BSerine/threonine-protein kinase aurora-B; aurora kinase B-Sv1; aurora kinase B-Sv2; Aurora/IPL1-related kinase 2; aurora-1; aurora-B; Aurora-related kinase 2; EC 2.7.11; IPL1; serine/threonine kinase 12; serine/threonine-protein kinase 12; STK12; STK12aurkb-sv1; STK5 AURKB AIK2, AIM-1, AIM1, ARK-2, ARK2, AurB, IPL1, PPP1R48, STK-1, STK12, STK5, aurkb-sv1, aurkb-sv2 aurora kinase B aurora kinase B|aurora kinase B-Sv1|aurora kinase B-Sv2|aurora- and Ipl1-like midbody-associated protein 1|aurora-1|aurora-B|aurora-related kinase 2|aurora/IPL1-related kinase 2|protein phosphatase 1, regulatory subunit 48|serine/threonine kinase 12|serine/threonine-protein kinase 12|serine/threonine-protein kinase 5|serine/threonine-protein kinase aurora-B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AURKB, check out the AURKB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AURKB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Aurora B AURKB Rabbit Monoclonal Antibody (M00762-1)
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Customer Q&As
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16 Customer Q&As for Anti-Aurora B AURKB Rabbit Monoclonal Antibody
Question
I was wanting to use using your anti-Aurora B Rabbit Monoclonal antibody for resolution of sister chromatid cohesion studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
I appreciate your inquiry. This M00762-1 anti-Aurora B Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IP, IF, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-21
Question
I am looking for to test anti-Aurora B Rabbit Monoclonal antibody M00762-1 on human lymph muscle for research purposes, then I may be interested in using anti-Aurora B Rabbit Monoclonal antibody M00762-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
The products we sell, including anti-Aurora B Rabbit Monoclonal antibody M00762-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-20
Question
We are currently using anti-Aurora B Rabbit Monoclonal antibody M00762-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
The anti-Aurora B Rabbit Monoclonal antibody (M00762-1) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-08
Question
Do you have a BSA free version of anti-Aurora B Rabbit Monoclonal antibody M00762-1 available?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Aurora B Rabbit Monoclonal antibody M00762-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-14
Question
Is a blocking peptide available for product anti-Aurora B Rabbit Monoclonal antibody (M00762-1)?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
We do provide the blocking peptide for product anti-Aurora B Rabbit Monoclonal antibody (M00762-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-17
Question
Is this M00762-1 anti-Aurora B Rabbit Monoclonal antibody reactive to the isotypes of AURKB?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
The immunogen of M00762-1 anti-Aurora B Rabbit Monoclonal antibody is A synthesized peptide derived from human Aurora B. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-16
Question
My question regarding product M00762-1, anti-Aurora B Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00762-1 anti-Aurora B Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-18
Question
I was wanting to use your anti-Aurora B Rabbit Monoclonal antibody for IP for human lymph muscle on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lymph muscle identification?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
You can see on the product datasheet, M00762-1 anti-Aurora B Rabbit Monoclonal antibody has been tested for IP, IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lymph muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-05
Question
We have observed staining in human liver spleen. Are there any suggestions? Is anti-Aurora B Rabbit Monoclonal antibody supposed to stain liver spleen positively?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
From what I have seen in literature liver spleen does express AURKB. From what I have seen in Uniprot.org, AURKB is expressed in oocyte, liver spleen, lung, lymph muscle, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have AURKB expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, and Spleen, Pubmed ID: 9858806
Lung, Lymph, and Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-07-12
Question
I see that the anti-Aurora B Rabbit Monoclonal antibody M00762-1 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-08
Question
My team were well pleased with the WB result of your anti-Aurora B Rabbit Monoclonal antibody. However we have seen positive staining in liver spleen nucleus. chromosome. chromosome using this antibody. Is that expected? Could you tell me where is AURKB supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-04-26
Answer
From what I have seen in literature, liver spleen does express AURKB. Generally AURKB expresses in nucleus. chromosome. chromosome, centromere. Regarding which tissues have AURKB expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, and Spleen, Pubmed ID: 9858806
Lung, Lymph, and Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-04-26
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymph muscle using anti-Aurora B Rabbit Monoclonal antibody M00762-1. Let me know if you need anything else.
S. Banerjee
Verified customer
Asked: 2018-01-17
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-17
Question
Please see the WB image, lot number and protocol we used for lymph muscle using anti-Aurora B Rabbit Monoclonal antibody M00762-1. Please let me know if you require anything else.
A. Thomas
Verified customer
Asked: 2016-11-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-11-09
Question
Will anti-Aurora B Rabbit Monoclonal antibody M00762-1 work for IP with lymph muscle?
J. Rodriguez
Verified customer
Asked: 2016-09-05
Answer
According to the expression profile of lymph muscle, AURKB is highly expressed in lymph muscle. So, it is likely that anti-Aurora B Rabbit Monoclonal antibody M00762-1 will work for IP with lymph muscle.
Boster Scientific Support
Answered: 2016-09-05
Question
Will M00762-1 anti-Aurora B Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
E. Kulkarni
Verified customer
Asked: 2015-11-11
Answer
You can see on the product datasheet, M00762-1 anti-Aurora B Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-11-11
Question
Our lab used your anti-Aurora B Rabbit Monoclonal antibody for WB on oocyte a few months ago. I am using human, and We intend to use the antibody for IHC next. We want examining oocyte as well as cervix carcinoma erythroleukemia in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
J. Rodriguez
Verified customer
Asked: 2013-05-15
Answer
I looked at the website and datasheets of our anti-Aurora B Rabbit Monoclonal antibody and it appears that M00762-1 has been tested on human in both WB and IHC. Thus M00762-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-05-15