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Product Info Summary
| SKU: | A01522 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-RPGR Antibody Picoband®
SKU/Catalog Number
A01522
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RPGR Antibody Picoband® catalog # A01522. Tested in ELISA, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RPGR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01522)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human RPGR recombinant protein (Position: A13-F338).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01522 is reactive to RPGR in Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
113 kDa
Calculated molecular weight
113.387kDa
Background of RPGR
X-linked retinitis pigmentosa GTPase regulator is a GTPase-binding protein that in humans is encoded by the RPGR gene. This gene encodes a protein with a series of six RCC1-like domains (RLDs), characteristic of the highly conserved guanine nucleotide exchange factors. The encoded protein is found in the Golgi body and interacts with RPGRIP1. This protein localizes to the outer segment of rod photoreceptors and is essential for their viability. Mutations in this gene have been associated with X-linked retinitis pigmentosa (XLRP). Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length natures of only some have been determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01522 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: rat liver tissue, rat kidney tissue, rat brain tissue, rat heart tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RPGR using anti-RPGR antibody (A01522).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPGR antigen affinity purified polyclonal antibody (Catalog # A01522) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPGR at approximately 113KD. The expected band size for RPGR is at 113KD.
Protein Target Info & Infographic
Gene/Protein Information For RPGR (Source: Uniprot.org, NCBI)
Gene Name
RPGR
Full Name
X-linked retinitis pigmentosa GTPase regulator
Weight
113.387kDa
Alternative Names
X-linked retinitis pigmentosa GTPase regulator; RPGR; RP3; XLRP3 RPGR COD1, CORDX1, CRD, PCDX, RP15, RP3, XLRP3, orf15 retinitis pigmentosa GTPase regulator X-linked retinitis pigmentosa GTPase regulator|retinitis pigmentosa 15|retinitis pigmentosa 3 GTPase regulator
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPGR, check out the RPGR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPGR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RPGR Antibody Picoband® (A01522)
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No publications found for A01522
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Customer Q&As
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7 Customer Q&As for Anti-RPGR Antibody Picoband®
Question
Is this A01522 anti-RPGR antibody reactive to the isotypes of RPGR?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
The immunogen of A01522 anti-RPGR antibody is E. coli-derived human RPGR recombinant protein (Position: A13-F338). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-29
Question
Will anti-RPGR antibody A01522 work for WB with retina?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
According to the expression profile of retina, RPGR is highly expressed in retina. So, it is likely that anti-RPGR antibody A01522 will work for WB with retina.
Boster Scientific Support
Answered: 2020-04-16
Question
We want to test anti-RPGR antibody A01522 on rat retina for research purposes, then I may be interested in using anti-RPGR antibody A01522 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
The products we sell, including anti-RPGR antibody A01522, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-17
Question
Do you have a BSA free version of anti-RPGR antibody A01522 available?
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-RPGR antibody A01522 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-23
Question
I have a question about product A01522, anti-RPGR antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-11-09
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01522 anti-RPGR antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-11-09
Question
We are currently using anti-RPGR antibody A01522 for rat tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says rat. Is it likely that the antibody can work on monkey tissues as well?
J. Moore
Verified customer
Asked: 2016-06-01
Answer
The anti-RPGR antibody (A01522) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-06-01
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for retina using anti-RPGR antibody A01522. Let me know if you need anything else.
O. Taylor
Verified customer
Asked: 2013-09-23
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-09-23
