Product Info Summary
SKU: | A00262 |
---|---|
Size: | 100ug |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-ATR Antibody
SKU/Catalog Number
A00262
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-ATR Antibody (Catalog # A00262). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-ATR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00262)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Standard Protein
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ATR protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00262 is reactive to ATR in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
301367 MW
Background of ATR
Ataxia Telangiectasia Mutated (ATM) and Rad 3-related protein (ATR) is a phosphatidyl-inositol kinase (PK)-related kinase which functions in response to DNA damage and repair as well as at DNA replication checkpoints during the cell cycle. ATR activates checkpoint signaling upon genotoxic stresses, such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. ATR is a member of the DNA-PK kinase family and is closely related to ATM and DNA-PK for which DNA stimulates the observed kinase activity. Chromosomal remodeling proteins have also been reported to associate with ATR complexes, including histone deacetylases (HDAC1, HDAC2 and CHD4). ATR is known to phosphorylate BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and TP53/p53 which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. ATR is a nuclear protein, but can also be found in PML nuclear bodies in certain cell types. ATR is recruited to chromatin during S-phase and redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00262 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:15,000 - 1:70,000
WB: 1:1,000 - 1:5,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of ATR expression in HELA cell nuclear extract (lane 1), MW markers (Lane M) and negligible staining after pre-incubation of antibody with the immunizing peptide (50 μg peptide for 1 h at room temperature followed by centrifugation) (lane 2). ATR at 301KD was detected using rabbit anti-ATR Antigen Affinity purified polyclonal antibody (Catalog # A00262) at 1:1400. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info & Infographic
Gene/Protein Information For ATR (Source: Uniprot.org, NCBI)
Gene Name
ATR
Full Name
Serine/threonine-protein kinase ATR
Weight
301367 MW
Superfamily
PI3/PI4-kinase family
Alternative Names
ataxia telangiectasia and Rad3 related; Ataxia telangiectasia and Rad3-related protein; ATR; EC 2.7.11.1; FRAP-related protein 1; FRP1; FRP1FRAP-related protein-1; MEC1; protein kinase ATR; Rad3 related protein; SCKL1; SCKLMEC1, mitosis entry checkpoint 1, homolog; serine/threonine-protein kinase ATR ATR FCTCS, FRP1, MEC1, SCKL, SCKL1 ATR serine/threonine kinase serine/threonine-protein kinase ATR|FRAP-related protein-1|MEC1, mitosis entry checkpoint 1, homolog|ataxia telangiectasia and Rad3-related protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATR, check out the ATR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATR Antibody (A00262)
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1 Customer Q&As for Anti-ATR Antibody
Question
We are currently using anti-ATR antibody A00262 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
The anti-ATR antibody (A00262) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-03