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Product Info Summary
| SKU: | PB9861 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-EGF Antibody Picoband®
SKU/Catalog Number
PB9861
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-EGF Antibody Picoband® catalog # PB9861. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EGF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9861)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human EGF recombinant protein (Position: N971-R1023). Human EGF shares 69.8% amino acid (aa) sequence identity with both mouse and rat EGF.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9861 is reactive to EGF in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
134 kDa
Calculated molecular weight
133994 MW
Background of EGF
EGF is known as epidermal growth factor. This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. Additionally, it acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9861 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: 22RV1 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of EGF using anti-EGF antibody (PB9861).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: 22RV1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGF antigen affinity purified polyclonal antibody (Catalog # PB9861) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGF at approximately 134 kDa. The expected band size for EGF is at 133 kDa.
Protein Target Info & Infographic
Gene/Protein Information For EGF (Source: Uniprot.org, NCBI)
Gene Name
EGF
Full Name
Pro-epidermal growth factor
Weight
133994 MW
Alternative Names
Pro-epidermal growth factor;EGF;Epidermal growth factor;Urogastrone;EGF; EGF HOMG4, URG epidermal growth factor pro-epidermal growth factor|beta-urogastrone
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EGF, check out the EGF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EGF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EGF Antibody Picoband® (PB9861)
Hello CJ!
PB9861 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
sEcad and EGF Levels Increased in Urine of Non-ferrous Metal Workers and Medium of Uroepithelial Cell Line Treated by Arsenic
Therapeutic Effect and Mechanism of Oxytropis falcata Gel on Deep Second-Degree Burn in Rats
Effect of IL-10 on the expression of HSC growth factors in hepatic fibrosis rat
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Customer Q&As
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16 Customer Q&As for Anti-EGF Antibody Picoband®
Question
Is a blocking peptide available for product anti-EGF antibody (PB9861)?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
We do provide the blocking peptide for product anti-EGF antibody (PB9861). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-01
Question
We have been able to see staining in human colon. Are there any suggestions? Is anti-EGF antibody supposed to stain colon positively?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
According to literature colon does express EGF. According to Uniprot.org, EGF is expressed in renal medulla, kidney, teratocarcinoma, colon, among other tissues. Regarding which tissues have EGF expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Kidney, Pubmed ID: 3491360
Teratocarcinoma, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-03-13
Question
Would anti-EGF antibody PB9861 work for ELISA with renal medulla?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
According to the expression profile of renal medulla, EGF is highly expressed in renal medulla. So, it is likely that anti-EGF antibody PB9861 will work for ELISA with renal medulla.
Boster Scientific Support
Answered: 2019-12-20
Question
We are currently using anti-EGF antibody PB9861 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
The anti-EGF antibody (PB9861) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-06
Question
Please see the WB image, lot number and protocol we used for renal medulla using anti-EGF antibody PB9861. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-20
Question
We purchased anti-EGF antibody for ELISA on kidney in a previous project. I am using human, and We are going to use the antibody for WB next. I am interested in examining kidney as well as colon in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
I have checked the website and datasheets of our anti-EGF antibody and it seems that PB9861 has been tested on human in both ELISA and WB. Thus PB9861 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-16
Question
We were content with the WB result of your anti-EGF antibody. However we have observed positive staining in colon membrane using this antibody. Is that expected? Could you tell me where is EGF supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
From literature, colon does express EGF. Generally EGF expresses in membrane. Regarding which tissues have EGF expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Kidney, Pubmed ID: 3491360
Teratocarcinoma, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-10-11
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for renal medulla using anti-EGF antibody PB9861. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-26
Question
I am interested in using your anti-EGF antibody for mapk cascade studies. Has this antibody been tested with western blotting on 22rv1 whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
Thanks for your inquiry. This PB9861 anti-EGF antibody is tested on 22rv1 whole cell lysates. It is guaranteed to work for ELISA, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-06-26
Question
Would PB9861 anti-EGF antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-04-16
Answer
You can see on the product datasheet, PB9861 anti-EGF antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-16
Question
I was wanting to use your anti-EGF antibody for ELISA for human renal medulla on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human renal medulla identification?
Verified Customer
Verified customer
Asked: 2018-12-14
Answer
It shows on the product datasheet, PB9861 anti-EGF antibody has been tested for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human renal medulla in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-14
Question
I see that the anti-EGF antibody PB9861 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-02-19
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-02-19
Question
Is there a BSA free version of anti-EGF antibody PB9861 available?
M. Miller
Verified customer
Asked: 2017-09-11
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-EGF antibody PB9861 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-09-11
Question
I have a question about product PB9861, anti-EGF antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
O. Jackson
Verified customer
Asked: 2017-06-30
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9861 anti-EGF antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-30
Question
We want to test anti-EGF antibody PB9861 on human renal medulla for research purposes, then I may be interested in using anti-EGF antibody PB9861 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
F. Williams
Verified customer
Asked: 2014-11-24
Answer
The products we sell, including anti-EGF antibody PB9861, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-11-24
Question
Is this PB9861 anti-EGF antibody reactive to the isotypes of EGF?
S. Edwards
Verified customer
Asked: 2014-02-27
Answer
The immunogen of PB9861 anti-EGF antibody is E. coli-derived human EGF recombinant protein (Position: N971-R1023). Human EGF shares 69.8% amino acid (aa) sequence identity with both mouse and rat EGF. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-02-27
