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Product Info Summary
| SKU: | A00362 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, ICC, WB |
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Product info
Product Name
Anti-ASC PYCARD Antibody
SKU/Catalog Number
A00362
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ASC PYCARD Antibody (Catalog # A00362). Tested in ELISA, WB, ICC, IHC-P applications. This antibody reacts with Human.
Storage & Handling
ASC antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ASC PYCARD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00362)
Host
Rabbit
Contents
ASC Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-ASC antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human ASC. The immunogen is located within the last 50 amino acids of ASC.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms of APG7 are known to exist; this antibody will detect all three isoforms. APG7 antibody is predicted not to cross-react with other ATG family proteins.
Reactive Species
A00362 is reactive to PYCARD in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
21627 MW
Background of ASC/TMS1
Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK, BCL10, Apaf-1, ARC, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified in human and mouse and designated ASC and TMS1. Ectopic expression of ASC/TMS1 induced apoptosis through activation of caspase-9 and inhibited the survival of human breast cancer cells (3, 4). Overexpression of ASC/TMS1 induced DNA fragmentation. ASC/TMS1 is expressed in a variety of human and mouse tissues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00362 is guaranteed for ELISA, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1-2 μg/mL; ICC: 5 μg/mL; IHC-P: 2.5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western Blot Validation in Human HL60 Cells
Loading: 15 μg of lysates per lane.Antibodies: ASC A00362, (1 μg/mL) in the absence (A) or presence (B) of blocking peptide, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: ASC A00362, (2 μg/mL), ASC 39-001, (2 μg/mL), beta-actin (1 μg/mL) and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit or goat anti-mouse IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Human THP-1 Cells
Loading: 15 μg of lysate per lane. Antibodies: ASC A00362, (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunohistochemistry Validation of ASC in Human Spleen Tissue
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ASC antibody (A00362) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunofluorescence Validation of ASC in Human Spleen Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Spleen Tissue labeling ASC with A00362 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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Immunocytochemistry Validation of ASC in HL60 Cells
Immunocytochemical analysis of HL60 cells using anti-ASC antibody (A00362) at 5 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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KD Validation of ASC in THP-1 Cells (Dowds et al., 2004)
Immunofluorescence analysis with anti-ASC antibodies (A00362) was performed for BIM in 293 cells transfected with GFP siRNA or ASC siRNA. ASC expression was disrupted after ASC siRNA knockdown.
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Overexpression Validation of ASC in HEK293T Cells (Dowds et al., 2004)
Western blot analysis with anti-ASC antibodies (A00362) was performed for ASC in HEK293T cells transfected with pcDNA3-ASC.
Protein Target Info & Infographic
Gene/Protein Information For PYCARD (Source: Uniprot.org, NCBI)
Gene Name
PYCARD
Full Name
Apoptosis-associated speck-like protein containing a CARD
Weight
21627 MW
Alternative Names
ASC, TMS, TMS1, CARD5, TMS-1, ASC, Apoptosis-associated speck-like protein containing a CARD, Caspase recruitment domain-containing protein 5, hASC PYCARD ASC, CARD5, TMS, TMS-1, TMS1 PYD and CARD domain containing apoptosis-associated speck-like protein containing a CARD|caspase recruitment domain-containing protein 5|target of methylation-induced silencing 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PYCARD, check out the PYCARD Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PYCARD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ASC PYCARD Antibody (A00362)
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No publications found for A00362
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