Product Info Summary
SKU: | A07192-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-ARP2/3 subunit 1B/ARPC1B Antibody Picoband®
View all actin-related protein 2/3 complex subunit 1B Antibodies
SKU/Catalog Number
A07192-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ARP2/3 subunit 1B/ARPC1B Antibody Picoband® catalog # A07192-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ARP2/3 subunit 1B/ARPC1B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07192-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ARP2/3 subunit 1B/ARPC1B recombinant protein (Position: Q293-K372).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07192-2 is reactive to ARPC1B in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
41 kDa
Calculated molecular weight
25503 MW
Background of actin-related protein 2/3 complex subunit 1B
Actin-related protein 2/3 complex subunit 1B is a protein that in humans is encoded by the ARPC1B gene. It is mapped to 7q22.1. This gene encodes one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1A. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages. This protein also has a role in centrosomal homeostasis by being an activator and substrate of the Aurora A kinase.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07192-2 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, Human
Positive Control
WB: human placenta issue, human PC-3 whole cell, human U-937 whole cell, rat spleen issue, mouse spleen issue
IHC: human tonsil tissue, mouse spleen tissue, rat spleen tissue
FCM: CACO-2 cell, PC-3 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Flow Cytometry analysis of CACO-2 cells using anti-ARPC1B antibody (A07192-2).
Overlay histogram showing CACO-2 cells stained with A07192-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARPC1B Antibody (A07192-2,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Click image to see more details
Figure 2. Flow Cytometry analysis of PC-3 cells using anti-ARPC1B antibody (A07192-2).
Overlay histogram showing PC-3 cells stained with A07192-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARPC1B Antibody (A07192-2,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Click image to see more details
Figure 3. IHC analysis of ARPC1B using anti-ARPC1B antibody (A07192-2).
ARPC1B was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ARPC1B Antibody (A07192-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of ARPC1B using anti-ARPC1B antibody (A07192-2).
ARPC1B was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ARPC1B Antibody (A07192-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of ARPC1B using anti-ARPC1B antibody (A07192-2).
ARPC1B was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ARPC1B Antibody (A07192-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. Western blot analysis of ARPC1B using anti-ARPC1B antibody (A07192-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta issue lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human U-937 whole cell lysates,
Lane 4: rat spleen issue lysates,
Lane 5: mouse spleen issue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARPC1B antigen affinity purified polyclonal antibody (Catalog # A07192-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARPC1B at approximately 41KD. The expected band size for ARPC1B is at 41KD.
Protein Target Info & Infographic
Gene/Protein Information For ARPC1B (Source: Uniprot.org, NCBI)
Gene Name
ARPC1B
Full Name
Actin-related protein 2/3 complex subunit 1B
Weight
25503 MW
Superfamily
WD repeat ARPC1 family
Alternative Names
Actin-related protein 2/3 complex subunit 1B; Arp2/3 complex 41 kDa subunit; p41-ARC; ARPC1B; ARC41 ARPC1B ARC41, IMD71, PLTEID, p40-ARC, p41-ARC actin related protein 2/3 complex subunit 1B actin-related protein 2/3 complex subunit 1B|ARP2/3 protein complex subunit p41|actin related protein 2/3 complex, subunit 1B, 41kDa|arp2/3 complex 41 kDa subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ARPC1B, check out the ARPC1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ARPC1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ARP2/3 subunit 1B/ARPC1B Antibody Picoband® (A07192-2)
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Customer Q&As
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1 Customer Q&As for Anti-ARP2/3 subunit 1B/ARPC1B Antibody Picoband®
Question
We are currently using anti-ARP2/3 subunit 1B/ARPC1B antibody A07192-2 for rat tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
D. Carter
Verified customer
Asked: 2014-10-28
Answer
The anti-ARP2/3 subunit 1B/ARPC1B antibody (A07192-2) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-10-28