Product Info Summary
SKU: | A01487 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-ARHGEF5 Antibody
SKU/Catalog Number
A01487
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ARHGEF5 Antibody catalog # A01487. Tested in WB applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ARHGEF5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01487)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human FEN1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01487 is reactive to ARHGEF5 in Human, Mouse, Rat
Applications
A01487 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
176799 MW
Background of ARHGEF5
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Murray J.M., Mol. Cell. Biol. 14:4878-4888(1994).
Hiraoka L.R., Genomics 25:220-225(1995).
Taylor T.D., Nature 440:497-500(2006).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
Validation Images & Assay Conditions
![a01487 anti arhgef5 antibody wb testing 1 a01487 anti arhgef5 antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a01487-anti-arhgef5-antibody-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blotting validation for Anti-ARHGEF5 Antibody A01487
Western blot (WB) analysis of ARHGEF5 polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysate
Lane2:sp2/0 cell lysate
Lane3:H9C2 cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For ARHGEF5 (Source: Uniprot.org, NCBI)
Gene Name
ARHGEF5
Full Name
Rho guanine nucleotide exchange factor 5
Weight
176799 MW
Alternative Names
ephexin-3; GEF5; Guanine nucleotide regulatory protein TIM; Oncogene TIM; p60 TIM; P60; Rho guanine nucleotide exchange factor (GEF) 5; rho guanine nucleotide exchange factor 5; TIM1; TIMDKFZp686N1969; Transforming immortalized mammary oncogene ARHGEF5 GEF5, P60, TIM, TIM1 Rho guanine nucleotide exchange factor 5 rho guanine nucleotide exchange factor 5|Rho guanine nucleotide exchange factor (GEF) 5|ephexin-3|ephexin3|guanine nucleotide regulatory protein TIM|oncogene TIM|p60 TIM|transforming immortalized mammary oncogene
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ARHGEF5, check out the ARHGEF5 Infographic
![ARHGEF5 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ARHGEF5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ARHGEF5 Antibody (A01487)
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No publications found for A01487
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