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Product Info Summary
| SKU: | EK0457 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse CCL19/MIP-3 beta ELISA Kit PicoKine®
View all CCL19/MIP-3 beta ELISA kits
SKU/Catalog Number
EK0457
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse CCL19/MIP-3 beta ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ccl19 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse CCL19/MIP-3 beta ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0457)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: G26-S108
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0457 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0457 is reactive to Ccl19 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0457 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CCL19/MIP-3 beta
Chemokine (C-C motif) ligand 19 (CCL19) is a small cytokine belonging to the CC chemokine family that is also known as EBI1 ligand chemokine (ELC) and macrophage inflammatory protein-3-beta (MIP-3-beta). Using PCR of human-rodent hybrids, the CCL19 gene was mapped to human chromosome 9p13, and the CCL19 gene is not part of the CC chemokine gene cluster on chromosome 17. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0457-CAP | Anti-Mouse Ccl19 Pre-coated 96-well strip microplate | 1 |
| EK0457-ST | Mouse Ccl19 Standard | 2 vials, 10 ng/tube |
| EK0457-DA | Mouse Ccl19 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.011 | 0.037 | 0.073 | 0.122 | 0.270 | 0.644 | 1.442 | 2.017 |
Data Example Images
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Mouse CCL19 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse CCL19/MIP-3 beta ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 30 | 161 | 384 | 27 | 175 | 349 |
| Standard deviation | 1.2 | 8.53 | 29.56 | 1.45 | 9.27 | 33.5 |
| CV (%) | 4% | 5.3% | 7.7% | 5.4% | 5.3% | 9.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CCL19/MIP-3 beta in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 30 | 32 | 28 | 32 | 30 | 1.65 | 5.5% |
| Sample 2 | 161 | 166 | 173 | 167 | 166 | 4.26 | 2.5% |
| Sample 3 | 384 | 401 | 402 | 407 | 398 | 8.67 | 2.1% |
Protein Target Info & Infographic
Gene/Protein Information For Ccl19 (Source: Uniprot.Org, NCBI)
Gene Name
Ccl19
Full Name
C-C motif chemokine 19
Weight
11911 MW
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
C-C motif chemokine 19;Epstein-Barr virus-induced molecule 1 ligand chemokine;EBI1 ligand chemokine;ELC;Small-inducible cytokine A19;Ccl19;Elc, Scya19; CCL19 CKb11, ELC, MIP-3b, MIP3B, SCYA19 C-C motif chemokine ligand 19 C-C motif chemokine 19|CC chemokine ligand 19|CK beta-11|EBI1-ligand chemokine|MIP-3-beta|beta chemokine exodus-3|chemokine (C-C motif) ligand 19|epstein-Barr virus-induced molecule 1 ligand chemokine|exodus-3|macrophage inflammatory protein 3-beta|small inducible cytokine subfamily A (Cys-Cys), member 19|small-inducible cytokine A19
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Ccl19, check out the Ccl19 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Ccl19: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse CCL19/MIP-3 beta ELISA Kit PicoKine® (EK0457)
Hello CJ!
EK0457 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Gut microbiota%u2013dependent modulation of innate immunity and lymph node remodeling affects cardiac allograft outcomes
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Mouse CCL19/MIP-3 beta ELISA Kit PicoKine®
Question
Does EK0457 contain native human biological materials?
Verified customer
Asked: 2021-11-10
Answer
The Mouse CCL19/MIP-3 Beta ELISA Kit PicoKine® (EK0457) doesn't contain native human biological materials.
Boster Scientific Support
Answered: 2021-11-11
Question
Q: is it okay to use citrate plasma as samples in Mouse CCL19 Picokine® ELISA Kit (Catalog # EK0457)?
B. Kaur
Verified customer
Asked: 2020-07-06
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of CCL19 causing degradation of its protein structure. CCL19 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the CCL19 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-07-06
Question
Q: Can CCL19 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-05-30
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-05-30
Question
Q: if the enzyme conjugated CCL19 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CCL19 antigen?
K. Wu
Verified customer
Asked: 2020-03-21
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-03-21
Question
Q: What is the optimal O.D. value for CCL19 ELISA kit? I performed your CCL19 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain CCL19 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2016-12-30
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CCL19 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CCL19 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2016-12-30
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2016-06-04
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2016-06-04
Question
Q: how to thaw whole blood sample for CCL19 ELISA after freezing?
G. Lopez
Verified customer
Asked: 2014-07-13
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CCL19 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2014-07-13
