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Product Info Summary
| SKU: | M00745-4 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Cyclin B1 Rabbit Monoclonal Antibody, Clone#RM281
SKU/Catalog Number
M00745-4
Size
100uL
Form
Liquid
Description
Boster Bio Anti-Cyclin B1 Rabbit Monoclonal Antibody, Clone#RM281 (Catalog # M00745-4). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyclin B1 Rabbit Monoclonal Antibody, Clone#RM281 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00745-4)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM281
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to Cyclin B1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human Cyclin B1.
Reactive Species
M00745-4 is reactive to CCNB1 in Human
Reconstitution
Calculated molecular weight
48337 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00745-4 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:1000-1:2000 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of Jurkat cell lysates using Anti-Cyclin B1 Rabbit Monoclonal Antibody (clone RM281) at a 1:1000 dilution.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Cyclin B1 Rabbit Monoclonal Antibody (clone RM281) at a 1:2000 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CCNB1 (Source: Uniprot.org, NCBI)
Gene Name
CCNB1
Full Name
G2/mitotic-specific cyclin-B1
Weight
48337 MW
Superfamily
cyclin family
Alternative Names
G2/mitotic-specific cyclin-B1;CCNB1;CCNB; CCNB1 CCNB cyclin B1 G2/mitotic-specific cyclin-B1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCNB1, check out the CCNB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCNB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cyclin B1 Rabbit Monoclonal Antibody, Clone#RM281 (M00745-4)
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No publications found for M00745-4
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Customer Q&As
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1 Customer Q&As for Anti-Cyclin B1 Rabbit Monoclonal Antibody, Clone#RM281
Question
We are currently using anti-Cyclin B1 Rabbit Monoclonal antibody, Clone#RM281 M00745-4 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
The anti-Cyclin B1 Rabbit Monoclonal antibody, Clone#RM281 (M00745-4) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-21
