Product Info Summary
SKU: | A01416 |
---|---|
Size: | 1mg |
Reactive Species: | Human |
Host: | Goat |
Application: | IHC |
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Product info
Product Name
Anti-APOLIPOPROTEINC-III APOC3 Antibody
View all Apolipoprotein CIII Antibodies
SKU/Catalog Number
A01416
Size
1mg
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-APOLIPOPROTEINC-III APOC3 Antibody (Catalog # A01416). Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store vial at 4°C prior to opening. This product is stable 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-APOLIPOPROTEINC-III APOC3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01416)
Host
Goat
Contents
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
produced from apoLipoprotein Type C-III derived from human plasma
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
A01416 is reactive to APOC3 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
68 kDa
Calculated molecular weight
154537 MW
Background of Apolipoprotein CIII
Anti Apolipoprotein C-III antibody recognizes the gene product of APOC. Apolipoprotein C-III is a protein component of very low density lipoprotein (VLDL). APOC3 inhibits lipoprotein lipase and hepatic lipase; it is thought to inhibit hepatic uptake of triglyceride-rich particles. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the A-1 and C-III genes are convergently transcribed. An increase in apoC-III levels induces the development of hypertriglyceridemia. This antibody is suitable for cardiovascular research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01416 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:10,000
IHC: 1:50 - 1:200
IP: 1:100
WB: 1:500 - 1:5,000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. IHC analysis of APOC3 using anti-APOC3 antibody (A01416).
APOC3 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-APOC3 Antibody (A01416) overnight at 4°C. Biotinylated goat anti Goat IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1023) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For APOC3 (Source: Uniprot.org, NCBI)
Gene Name
APOC3
Full Name
Apolipoprotein C-III
Weight
154537 MW
Superfamily
apolipoprotein C3 family
Alternative Names
Apolipoprotein C-III, ApoC-III, Apo-CIII, Apolipoprotein C3, Apolipoprotein C III APOC3 APOCIII apolipoprotein C3 apolipoprotein C-III
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on APOC3, check out the APOC3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for APOC3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-APOLIPOPROTEINC-III APOC3 Antibody (A01416)
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No publications found for A01416
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Customer Q&As
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4 Customer Q&As for Anti-APOLIPOPROTEINC-III APOC3 Antibody
Question
I see that the anti-APOLIPOPROTEINC-III antibody A01416 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-08
Question
I have a question about product A01416, anti-APOLIPOPROTEINC-III antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01416 anti-APOLIPOPROTEINC-III antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-26
Question
Please see the WB image, lot number and protocol we used for liver using anti-APOLIPOPROTEINC-III antibody A01416. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-06-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-12
Question
Is this A01416 anti-APOLIPOPROTEINC-III antibody reactive to the isotypes of APOC3?
R. Anderson
Verified customer
Asked: 2015-03-12
Answer
The immunogen of A01416 anti-APOLIPOPROTEINC-III antibody is produced from apoLipoprotein Type C-III derived from human plasma. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-03-12