Product Info Summary
SKU: | PA2075 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Angiopoietin-related protein 3 ANGPTL3 Antibody Picoband®
View all Angiopoietin-like Protein 3/ANGPTL3 Antibodies
SKU/Catalog Number
PA2075
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Angiopoietin-related protein 3 ANGPTL3 Antibody catalog # PA2075. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Angiopoietin-related protein 3 ANGPTL3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2075)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human ANGPTL3, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2075 is reactive to ANGPTL3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
50 kDa
Calculated molecular weight
53637 MW
Background of Angiopoietin-like Protein 3/ANGPTL3
ANGPTL3 (Angiopoietin-Like 3), also known as ANGPT5, is a protein which in humans is encoded by the ANGPTL3 gene. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. By radiation hybrid mapping and the use of surrounding genes, Conklin et al. (1999) mapped the ANGPTL3 gene to chromosome 1p31. It is predominantly expressed in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain and the C-terminal fibrinogen (FBN)-like domain. Angptl3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. ANGPTL3 inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2075 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: A549 Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-ANGPTL3 antibody, PA2075, Western blotting
WB: A549 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For ANGPTL3 (Source: Uniprot.org, NCBI)
Gene Name
ANGPTL3
Full Name
Angiopoietin-related protein 3
Weight
53637 MW
Alternative Names
Angiopoietin-related protein 3;Angiopoietin-5;ANG-5;Angiopoietin-like protein 3;ANGPTL3;ANGPT5;UNQ153/PRO179; ANGPTL3 ANG-5, ANGPT5, ANL3, FHBL2 angiopoietin like 3 angiopoietin-related protein 3|angiopoietin 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ANGPTL3, check out the ANGPTL3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ANGPTL3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Angiopoietin-related protein 3 ANGPTL3 Antibody Picoband® (PA2075)
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No publications found for PA2075
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Customer Q&As
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4 Customer Q&As for Anti-Angiopoietin-related protein 3 ANGPTL3 Antibody Picoband®
Question
We have been able to see staining in rat plasma. Do you have any suggestions? Is anti-ANGPTL3 antibody supposed to stain plasma positively?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
According to literature plasma does express ANGPTL3. According to Uniprot.org, ANGPTL3 is expressed in liver, liver skeletal muscle, plasma, among other tissues. Regarding which tissues have ANGPTL3 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 10644446, 14702039, 19159218
Liver, and Skeletal muscle, Pubmed ID: 15489334
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2020-04-16
Question
We are currently using anti-ANGPTL3 antibody PA2075 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
The anti-ANGPTL3 antibody (PA2075) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-22
Question
My colleagues were well pleased with the WB result of your anti-ANGPTL3 antibody. However we have seen positive staining in liver skeletal muscle secreted using this antibody. Is that expected? Could you tell me where is ANGPTL3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-09-27
Answer
From what I have seen in literature, liver skeletal muscle does express ANGPTL3. Generally ANGPTL3 expresses in secreted. Regarding which tissues have ANGPTL3 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 10644446, 14702039, 19159218
Liver, and Skeletal muscle, Pubmed ID: 15489334
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2018-09-27
Question
I am looking for using your anti-ANGPTL3 antibody for integrin-mediated signaling pathway studies. Has this antibody been tested with western blotting on a549 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-12-18
Answer
We appreciate your inquiry. This PA2075 anti-ANGPTL3 antibody is validated on a549 cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-12-18