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Product Info Summary
| SKU: | P00007-3 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal Antibody, Clone#RM261
SKU/Catalog Number
P00007-3
Size
100uL
Form
Liquid
Description
Boster Bio Anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal Antibody, Clone#RM261 (Catalog # P00007-3). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal Antibody, Clone#RM261 (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00007-3)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM261
Isotype
Rabbit IgG
Immunogen
A phospho-peptide corresponding to human Phospho- Stat3 (Tyr705)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human Stat3 only when phosphorylated at Tyr705. There is no cross-reactivity to stat3 without phosphorylation at Tyr705. This antibody may also react to mouse or rat Phospho-Stat3 (Tyr705), as predicted by immunogen homology.
Reactive Species
P00007-3 is reactive to STAT3 in Human
Reconstitution
Calculated molecular weight
88068 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00007-3 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:1000-1:10,000 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of A431 cell lysates, nontreated (-) or treated (+) with EGF, using Anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal Antibody (clone RM261) at a 1:1000 dilution, showed Phospho-Stat3 (Tyr705) in EGF-treated A431 cells.
Click image to see more details
Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal Antibody (clone RM261) at a 1:10,000 dilution.
Protein Target Info & Infographic
Gene/Protein Information For STAT3 (Source: Uniprot.org, NCBI)
Gene Name
STAT3
Full Name
Signal transducer and activator of transcription 3
Weight
88068 MW
Superfamily
transcription factor STAT family
Alternative Names
Signal transducer and activator of transcription 3 ;Acute-phase response factor;STAT3 ;APRF ; STAT3 ADMIO, ADMIO1, APRF, HIES signal transducer and activator of transcription 3 signal transducer and activator of transcription 3|DNA-binding protein APRF|acute-phase response factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STAT3, check out the STAT3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STAT3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal Antibody, Clone#RM261 (P00007-3)
Hello CJ!
P00007-3 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Protective effects of Tongxinluo on cerebral ischemia/reperfusion injury related to Connexin 43/Calpain II/Bax/Caspase-3 pathway in rat
IL-6 Inhibits Starvation-induced Autophagy via the STAT3/Bcl-2 Signaling Pathway
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal Antibody, Clone#RM261
Question
I was wanting to use your anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal antibody, Clone#RM261 for WB for human erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
It shows on the product datasheet, P00007-3 anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal antibody, Clone#RM261 has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-22
Question
Our lab used your anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
I looked at the website and datasheets of our anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2020-04-07
Question
Is a blocking peptide available for product anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal antibody, Clone#RM261 (P00007-3)?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
We do provide the blocking peptide for product anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal antibody, Clone#RM261 (P00007-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-25
Question
See attached the WB image, lot number and protocol we used for erythroleukemia using anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal antibody, Clone#RM261 P00007-3. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-19
Question
We have seen staining in mouse upper lobe of lung. What should we do? Is anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal antibody, Clone#RM261 supposed to stain upper lobe of lung positively?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
From literature upper lobe of lung does express STAT3. From Uniprot.org, STAT3 is expressed in upper lobe of lung, placenta, kidney pancreas, liver, cervix carcinoma, erythroleukemia, among other tissues. Regarding which tissues have STAT3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Erythroleukemia, Pubmed ID: 23186163
Kidney, and Pancreas, Pubmed ID: 15489334
Liver, Pubmed ID: 7701321, 24275569
Placenta, Pubmed ID: 7512451
Boster Scientific Support
Answered: 2020-01-14
Question
Do you have a BSA free version of anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal antibody, Clone#RM261 P00007-3 available?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal antibody, Clone#RM261 P00007-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-06
Question
Is this P00007-3 anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal antibody, Clone#RM261 reactive to the isotypes of STAT3?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
The immunogen of P00007-3 anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal antibody, Clone#RM261 is A phospho-peptide corresponding to human Phospho- Stat3 (Tyr705). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-15
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal antibody, Clone#RM261 P00007-3. Let me know if you need anything else.
N. Brown
Verified customer
Asked: 2019-09-17
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-17
Question
We were content with the WB result of your anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
Based on literature, erythroleukemia does express STAT3. Generally STAT3 expresses in cytoplasm. nucleus. Regarding which tissues have STAT3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Erythroleukemia, Pubmed ID: 23186163
Kidney, and Pancreas, Pubmed ID: 15489334
Liver, Pubmed ID: 7701321, 24275569
Placenta, Pubmed ID: 7512451
Boster Scientific Support
Answered: 2019-08-14
Question
I have a question about product P00007-3, anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal antibody, Clone#RM261. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-02-19
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P00007-3 anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal antibody, Clone#RM261, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-02-19
Question
We are currently using anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal antibody, Clone#RM261 P00007-3 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-13
Answer
The anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal antibody, Clone#RM261 (P00007-3) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-13
Question
Would P00007-3 anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal antibody, Clone#RM261 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
L. Miller
Verified customer
Asked: 2017-10-31
Answer
As indicated on the product datasheet, P00007-3 anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal antibody, Clone#RM261 as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-31
Question
Will anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal antibody, Clone#RM261 P00007-3 work for WB with erythroleukemia?
Verified Customer
Verified customer
Asked: 2017-08-01
Answer
According to the expression profile of erythroleukemia, STAT3 is highly expressed in erythroleukemia. So, it is likely that anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal antibody, Clone#RM261 P00007-3 will work for WB with erythroleukemia.
Boster Scientific Support
Answered: 2017-08-01
Question
I see that the anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal antibody, Clone#RM261 P00007-3 works with WB, what is the protocol used to produce the result images on the product page?
V. Mitchell
Verified customer
Asked: 2016-04-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-04-25
Question
you antibody using your anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal antibody, Clone#RM261 for ptk6 activates stat3 studies. Has this antibody been tested with western blotting on breast cancer tissue? We would like to see some validation images before ordering.
T. Zhang
Verified customer
Asked: 2016-02-17
Answer
We appreciate your inquiry. This P00007-3 anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal antibody, Clone#RM261 is validated on a431 cells, breast cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-02-17
Question
I was wanting to use to test anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal antibody, Clone#RM261 P00007-3 on human erythroleukemia for research purposes, then I may be interested in using anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal antibody, Clone#RM261 P00007-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
E. Taylor
Verified customer
Asked: 2015-06-02
Answer
The products we sell, including anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal antibody, Clone#RM261 P00007-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-06-02
