Product Info Summary
SKU: | PB9479 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Apg7/ATG7 Antibody Picoband®
SKU/Catalog Number
PB9479
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Apg7/ATG7 Antibody Picoband® catalog # PB9479. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Apg7/ATG7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9479)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Apg7, different from the related mouse and rat sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9479 is reactive to ATG7 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
78 kDa
Calculated molecular weight
77960 MW
Background of ATG7
Autophagy-related protein 7 is a protein that in humans is encoded by the ATG7 gene. It is mapped to 3p25.3. This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein also shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. ATG7 is essential for the Apg12 conjugation system that mediates membrane fusion in autophagy. It is found that when nutrients are limited, ATG7 can regulate p53-dependent cell cycle and cell death pathways.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9479 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Brain Tissue, Mouse Brain Tissue, 293T Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of APG7 using anti-APG7 antibody (PB9479).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Brain Tissue Lysate at 50ug,
Lane 2: Mouse Brain Tissue Lysate at 50ug,
Lane 3: 293T Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APG7 antigen affinity purified polyclonal antibody (Catalog # PB9479) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APG7 at approximately 78 kDa. The expected band size for APG7 is at 78 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ATG7 (Source: Uniprot.org, NCBI)
Gene Name
ATG7
Full Name
Ubiquitin-like modifier-activating enzyme ATG7
Weight
77960 MW
Superfamily
ATG7 family
Alternative Names
Ubiquitin-like modifier-activating enzyme ATG7;ATG12-activating enzyme E1 ATG7;Autophagy-related protein 7;APG7-like;hAGP7;Ubiquitin-activating enzyme E1-like protein;ATG7;APG7L; ATG7 APG7-LIKE, APG7L, GSA7 autophagy related 7 ubiquitin-like modifier-activating enzyme ATG7|APG7 autophagy 7-like|ATG12-activating enzyme E1 ATG7|hAGP7|ubiquitin-activating enzyme E1-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATG7, check out the ATG7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATG7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Apg7/ATG7 Antibody Picoband® (PB9479)
Hello CJ!
PB9479 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Erbin exerts a protective effect against inflammatory bowel disease by suppressing autophagic cell death
Nrf2 signalling andautophagyare involved in diabetes mellitus-induced defects in the development of mouse placenta
Yang J, Sheng S, Yang Q, Li L, Qin S, Yu S, Zhang X. Oncol Lett. 2017 Nov;14(5):5333-5339. doi: 10.3892/ol.2017.6857. Epub 2017 Aug 31. Endocan silencing induces programmed cell death in hepatocarcinoma
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Customer Q&As
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5 Customer Q&As for Anti-Apg7/ATG7 Antibody Picoband®
Question
We are currently using anti-Apg7/ATG7 antibody PB9479 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
The anti-Apg7/ATG7 antibody (PB9479) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-31
Question
Is a blocking peptide available for product anti-Apg7/ATG7 antibody (PB9479)?
L. Miller
Verified customer
Asked: 2019-12-12
Answer
We do provide the blocking peptide for product anti-Apg7/ATG7 antibody (PB9479). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-12
Question
Can you help my question with product PB9479, anti-Apg7/ATG7 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Baker
Verified customer
Asked: 2019-11-18
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9479 anti-Apg7/ATG7 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-18
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-Apg7/ATG7 antibody PB9479. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-11-26
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-26
Question
Is there a BSA free version of anti-Apg7/ATG7 antibody PB9479 available?
Z. Wu
Verified customer
Asked: 2016-05-27
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Apg7/ATG7 antibody PB9479 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-05-27