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Product Info Summary
| SKU: | PB9450 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Thrombospondin/THBS1 Antibody Picoband®
View all Thrombospondin-1 Antibodies
SKU/Catalog Number
PB9450
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Thrombospondin/THBS1 Antibody Picoband® catalog # PB9450. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Thrombospondin/THBS1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9450)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human THBS1, different from the related mouse sequence by five amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9450 is reactive to THBS1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
160 kDa
Calculated molecular weight
129 kDa
Background of Thrombospondin-1
THBS1 is also known as Thrombospondin 1, TSP1. The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. It is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Also this protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. In addition, the thrombospondin-1 protein is a member of the thrombospondinfamily. It is a multi-domain matrix glycoprotein that has been shown to be a natural inhibitor of neovascularization and tumorigenesis in healthy tissue. Both positive and negative modulation of endothelial cell adhesion, motility, and growth have been attributed to TSP1. This should not be surprising considering that TSP1 interacts with at least 12 cell adhesion receptors, including CD36, αv integrins, β1 integrins, syndecan, and integrin-associated protein (IAP or CD47). It also interacts with numerous proteases involved in angiogenesis, including plasminogen, urokinase, matrix metalloproteinase, thrombin, cathepsin, and elastase.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9450 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human HepG2 whole cell, human K562 whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of THBS1 using anti-THBS1 antibody (PB9450).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: rat C6 whole cell lysates,
Lane 4: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-THBS1 antigen affinity purified polyclonal antibody (Catalog # PB9450) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for THBS1 at approximately 160 kDa. The expected band size for THBS1 is at 129 kDa.
Protein Target Info & Infographic
Gene/Protein Information For THBS1 (Source: Uniprot.org, NCBI)
Gene Name
THBS1
Full Name
Thrombospondin-1
Weight
129 kDa
Superfamily
thrombospondin family
Alternative Names
Thrombospondin-1;THBS1;TSP, TSP1; THBS1 THBS, THBS-1, TSP, TSP-1, TSP1 thrombospondin 1 thrombospondin-1|glycoprotein G|thrombospondin-1p180|thrombospondin-p50
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on THBS1, check out the THBS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for THBS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Thrombospondin/THBS1 Antibody Picoband® (PB9450)
Hello CJ!
PB9450 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Reactive oxygen species%u2013mediated switching expression of MMP-3 in stromal fibroblasts and cancer cells during prostate cancer progression
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Customer Q&As
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6 Customer Q&As for Anti-Thrombospondin/THBS1 Antibody Picoband®
Question
I am looking for to test anti-Thrombospondin/THBS1 antibody PB9450 on human liver for research purposes, then I may be interested in using anti-Thrombospondin/THBS1 antibody PB9450 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
The products we sell, including anti-Thrombospondin/THBS1 antibody PB9450, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-03
Question
I see that the anti-Thrombospondin/THBS1 antibody PB9450 works with WB, what is the protocol used to produce the result images on the product page?
M. Roberts
Verified customer
Asked: 2019-07-29
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-29
Question
Can you help my question with product PB9450, anti-Thrombospondin/THBS1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9450 anti-Thrombospondin/THBS1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-31
Question
We are currently using anti-Thrombospondin/THBS1 antibody PB9450 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
S. Lewis
Verified customer
Asked: 2018-09-03
Answer
The anti-Thrombospondin/THBS1 antibody (PB9450) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-03
Question
I was wanting to use your anti-Thrombospondin/THBS1 antibody for WB for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
S. Patel
Verified customer
Asked: 2015-05-15
Answer
You can see on the product datasheet, PB9450 anti-Thrombospondin/THBS1 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-05-15
Question
Is a blocking peptide available for product anti-Thrombospondin/THBS1 antibody (PB9450)?
K. Krishna
Verified customer
Asked: 2014-02-27
Answer
We do provide the blocking peptide for product anti-Thrombospondin/THBS1 antibody (PB9450). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-02-27
