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Product Info Summary
| SKU: | A04573-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-APC6 CDC16 Antibody
SKU/Catalog Number
A04573-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-APC6 CDC16 Antibody (Catalog # A04573-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
APC6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-APC6 CDC16 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04573-1)
Host
Rabbit
Contents
APC6 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
APC6 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human APC6. The immunogen is located within the first 50 amino acids of APC6.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least five alternatively spliced transcript variants have been observed. COH1 detects two isoforms.
Reactive Species
A04573-1 is reactive to CDC16 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
71656 MW
Background of CDC16
Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC6 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC6, it has been shown to interact with and is stabilized by CDC26 through an intermolecular TPR mimic composed of one helix from each protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04573-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
APC6 antibody can be used for detection of APC6 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of APC6 in human liver tissue lysate with APC6 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of APC6 in human liver tissue with APC6 antibody at 5 μg/mL.
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Immunofluorescence of APC6 in human liver tissue with APC6 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For CDC16 (Source: Uniprot.org, NCBI)
Gene Name
CDC16
Full Name
Cell division cycle protein 16 homolog
Weight
71656 MW
Superfamily
APC6/CDC16 family
Alternative Names
APC6, CUT9, ANAPC6, Cell division cycle protein 16 homolog, Anaphase-promoting complex subunit 6, APC6 CDC16 ANAPC6, APC6Hs, CUT9, CDC16 cell division cycle 16 cell division cycle protein 16 homolog|anaphase-promoting complex, subunit 6|cell division cycle 16 homolog|cyclosome subunit 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDC16, check out the CDC16 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDC16: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-APC6 CDC16 Antibody (A04573-1)
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