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Product Info Summary
| SKU: | A08280-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-APC5 ANAPC5 Antibody
SKU/Catalog Number
A08280-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-APC5 ANAPC5 Antibody (Catalog # A08280-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
APC5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-APC5 ANAPC5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08280-1)
Host
Rabbit
Contents
APC5 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
APC5 antibody was raised against a 17 amino acid synthetic peptide near the center of human APC5. The immunogen is located within amino acids 480 - 530 of APC5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
SPRYD2 antibody is predicted to not cross-react with other SPRYD protein family members. At least four isoforms of SPRYD2 are known to exist.
Reactive Species
A08280-1 is reactive to ANAPC5 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
85077 MW
Background of Apc5
Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC5 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC5, it is thought that APC5 associates with other APC/C components APC1, APC4, and CDC23 interdependently, such that loss of any one subunit reduces binding between the remaining three.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08280-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
APC5 antibody can be used for detection of APC5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of APC5 in human kidney tissue lysate with APC5 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of APC5 in rat kidney tissue with APC5 antibody at 5 μg/mL.
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Immunofluorescence of APC5 in rat kidney tissue with APC5 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ANAPC5 (Source: Uniprot.org, NCBI)
Gene Name
ANAPC5
Full Name
Anaphase-promoting complex subunit 5
Weight
85077 MW
Superfamily
APC5 family
Alternative Names
APC5, APC5, Anaphase-promoting complex subunit 5, Cyclosome subunit 5 ANAPC5 APC5 anaphase promoting complex subunit 5 anaphase-promoting complex subunit 5|cyclosome subunit 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ANAPC5, check out the ANAPC5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ANAPC5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-APC5 ANAPC5 Antibody (A08280-1)
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