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- Table of Contents
Facts about Anaphase-promoting complex subunit 5.
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Human | |
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Gene Name: | ANAPC5 |
Uniprot: | Q9UJX4 |
Entrez: | 51433 |
Belongs to: |
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APC5 family |
anaphase promoting complex subunit 5; anaphase-promoting complex subunit 5; APC5Cyclosome subunit 5
Mass (kDA):
85.077 kDA
Human | |
---|---|
Location: | 12q24.31 |
Sequence: | 12; NC_000012.12 (121308245..121354209, complement) |
This article explains the ANAPC5 marker. It covers the Boster bio history, the high affinity primary antibodies, the methods for recording the test result with autoradiography film, as well as the Protein transfer efficiency using membrane staining. This article will help you to choose the best antibody for your ANAPC5 investigation.
Boster has a wide range of quality antibodies and reagents including rabbit polyclonal antibody and highly cited ELISA kit. Boster antibodies have been cited over 29,000 times and are optimized for ELISA/WB use. The company offers primary antibodies and secondary antibodies for a variety applications, including neuroscience. Boster offers a High-Affinity Quality Guarantee that guarantees that each antibody or reagent will work exactly as described.
High-affinity antibodies are highly specific, as they can wash off non-specific bound materials. Boster's primary antibodies with high-affinity use the ANAPC5 marker for detecting ANAP-G-related protein to maximize the accuracy of ELISA tests. High-affinity antibodies make it easy to measure specific analytes within crude preparations.
Using dual-antibodies for detection is particularly useful in cases where two antigens are detected on a tissue section. Both the antigens can be seen simultaneously or sequentially. However, their pigments will not interfere with the other antigens. Optic filter combinations can be used to selectively visualize both antigens, which limits cross-reactivity. Dual-antibody detection also requires an additional incubation step that increases the assay's sensitivity.
This antibody can also be used in immunoassays, diagnostic tests, and other procedures that use the ANAPC5 molecule marker. This antibody recognizes ANAPC5 protein in a consistent manner, and can detect a specific molecule on each specimen. Researchers can use the ANAPC5 marker to determine the percentage of certain cell types within a tissue.
Autoradiography is an image-recording method that uses radiolabelled compounds to determine the spatial distribution in biological specimens. Historically, autoradiography was only used in the medical field but is now also used for environmental sample analyses. The radioactive material is placed in a special emulsion which contains silver bromide. The samples are then exposed to the emulsion using standard photographic techniques, and the resulting image is obtained. You can view the autoradiography images microscopically as well as recording the image.
Boster Bio has created a new method that improves protein transport efficiency by increasing the ability of membranes for re-probe antibodies. In this method, proteins with different molecular weights are incubated on NC or PVDF membranes. After the membranes had been blocked with BSA or other antibodies, they were reprobed with different antibodies. The results indicated that the PVDF has better reprobing capabilities than its NC counterpart. The PVDF membrane demonstrated a better reprobing capability than the NC membrane for the exact same protein. Additionally, PVDF membrane is more able to bind glycoproteins to it than NC membrane. The advantages of PVDF membranes over NC membranes were also evident in the three other cases.
Semi-dry and dry protein transfer offer several benefits, including improved accessibility for antibodies or stains, and shorter transfer times. The most important difference is the amount and type of equipment needed. The most efficient transfer is achieved by choosing the method that best suits your protein concentration, membrane type, and time. Once you have chosen the method, you can rest assured that proteins will be transferred to the membrane effectively.
After using a pooled human serum sample, the resulting fraction contains about 10 mg of protein. The protein was separated using 10% SDS–PAGE. For staining, the membrane was electroblotted onto PVDF or Nitrocellulose membranes. The membrane was rinsed with 50% alcohol to remove the background. Depending on the PVDF or nitrocellulose membrane, the proteins will be visualized as black bars on a light or dark background.
Boster Bio western Blotting has been made more sensitive and reproducible, which in turn has improved protein transfer efficiency. It is now possible to detect proteins on PVDF and NC membranes with a much lower amount of serum proteins than before. Boster Bio Westernblotting also benefits from the use of better electrode materials and fixatives. Researchers are now able to perform protein transfer much more accurately thanks to these improvements.
The pore size of the membrane used for WB depends on the molecular weight of the proteins. For proteins below 20kD, a membrane of 0.2mm will perform better. CerP is a low molecular weight protein and requires a 0.2 mm membrane to transfer it. A PVDF membrane offers greater sensitivity, resolution and affinity for protein detection.
This page contains historical landmarks related to Steven Boster's life. We have public records of Steve Boster. This includes his home address and past addresses as well as email addresses. You can filter these records by age and state to find out more about Steve Boster. For background information on Steve's life, including his childhood and early career as an actor, read the following.
Steve Boster passed away on June 26, 2022. He was born in Joliet (IL). He was a long-standing retail salesman, a U.S. Army veteran, as well as a member at Concordia Hall in Staunton. His survivors include his 2 daughters, Natosha Pueck and Crystal Boster as well as 6 grandchildren and 4 brothers, Jack Boster Sandra Blanton, Tammy, and Tammy. He also leaves behind a number of nieces or nephews.
PMID: 9469815 by Yu H., et al. Identification of a cullin homology region in a subunit of the anaphase-promoting complex.
PMID: 14657031 by Kraft C., et al. Mitotic regulation of the human anaphase-promoting complex by phosphorylation.