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Product Info Summary
| SKU: | A00008 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-APC Antibody
SKU/Catalog Number
A00008
Size
100μl
Form
Liquid
Description
Boster Bio Anti-APC Antibody catalog # A00008. Tested in ELISA, IF, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-APC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00008)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human APC. AA range:2794-2843
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00008 is reactive to APC in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
311646 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00008 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Validation Images & Assay Conditions
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Western blot analysis of COLO205 cells using APC Polyclonal Antibody diluted at 1:500
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Western blot analysis of various cells using APC Polyclonal Antibody diluted at 1:500
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Western blot analysis of lysates from COLO205 cells, using APC Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using APC Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of Hela cell. 1, APC Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Galectin-3 monoclonal antibody (6G2) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).
Protein Target Info & Infographic
Gene/Protein Information For APC (Source: Uniprot.org, NCBI)
Gene Name
APC
Full Name
Adenomatous polyposis coli protein
Weight
311646 MW
Superfamily
adenomatous polyposis coli (APC) family
Alternative Names
Adenomatous polyposis coli protein;Protein APC;Deleted in polyposis 2.5;APC;DP2.5; APC BTPS2, DESMD, DP2, DP2.5, DP3, GS, PPP1R46 APC regulator of WNT signaling pathway adenomatous polyposis coli protein|APC, WNT signaling pathway regulator|WNT signaling pathway regulator|adenomatosis polyposis coli tumor suppressor|adenomatous polyposis coli (APC)|deleted in polyposis 2.5|epididymis secretory sperm binding protein|protein phosphatase 1, regulatory subunit 46|truncated adenomatosis polyposis coli
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on APC, check out the APC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for APC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-APC Antibody (A00008)
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Customer Q&As
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7 Customer Q&As for Anti-APC Antibody
Question
Will A00008 anti-APC antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
As indicated on the product datasheet, A00008 anti-APC antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-27
Question
I was wanting to use your anti-APC antibody for WB for human fetal brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human fetal brain identification?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
It shows on the product datasheet, A00008 anti-APC antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human fetal brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-17
Question
My question regarding product A00008, anti-APC antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00008 anti-APC antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-11
Question
Is this A00008 anti-APC antibody reactive to the isotypes of APC?
Verified Customer
Verified customer
Asked: 2019-04-11
Answer
The immunogen of A00008 anti-APC antibody is Synthesized peptide derived from the C-terminal region of human APC. at AA rangle: 2770-2850. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-04-11
Question
Would anti-APC antibody A00008 work on canine WB with pigmented layer of retina?
Verified Customer
Verified customer
Asked: 2017-12-28
Answer
Our lab technicians have not tested anti-APC antibody A00008 on canine. You can run a BLAST between canine and the immunogen sequence of anti-APC antibody A00008 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine pigmented layer of retina in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-28
Question
Is there a BSA free version of anti-APC antibody A00008 available?
Verified Customer
Verified customer
Asked: 2017-07-10
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-APC antibody A00008 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-07-10
Question
I see that the anti-APC antibody A00008 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-06-29
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-06-29
