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Product Info Summary
| SKU: | M00008 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-APC Monoclonal Antibody
SKU/Catalog Number
M00008
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-APC Monoclonal Antibody catalog # M00008. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-APC Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00008)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FAD-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human APC Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF) -induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00008 is reactive to APC in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
54 kDa
Calculated molecular weight
88068 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00008 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
IHC: human colon cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of APC expression in 293T cell lysate.
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Immunohistochemical analysis of paraffin-embedded human colon cancer, using APC Antibody.
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All lanes use the Antibody at 1:4K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For APC (Source: Uniprot.org, NCBI)
Gene Name
APC
Full Name
Adenomatous polyposis coli protein
Weight
88068 MW
Superfamily
adenomatous polyposis coli (APC) family
Alternative Names
Signal transducer and activator of transcription 3;Acute-phase response factor;STAT3;APRF; APC BTPS2, DESMD, DP2, DP2.5, DP3, GS, PPP1R46 APC regulator of WNT signaling pathway adenomatous polyposis coli protein|APC, WNT signaling pathway regulator|WNT signaling pathway regulator|adenomatosis polyposis coli tumor suppressor|adenomatous polyposis coli (APC)|deleted in polyposis 2.5|epididymis secretory sperm binding protein|protein phosphatase 1, regulatory subunit 46|truncated adenomatosis polyposis coli
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on APC, check out the APC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for APC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-APC Monoclonal Antibody (M00008)
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1 Customer Q&As for Anti-APC Monoclonal Antibody
Question
We are currently using anti-APC Monoclonal antibody M00008 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on pig tissues as well?
C. Bhatt
Verified customer
Asked: 2019-01-09
Answer
The anti-APC Monoclonal antibody (M00008) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-09
