Click image to see more details
Product Info Summary
| SKU: | A00024-1 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-AKT AKT1 Antibody
SKU/Catalog Number
A00024-1
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-AKT AKT1 Antibody (Catalog # A00024-1). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-AKT AKT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00024-1)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-AKT Pan Reactive Antibody was produced in rabbits by repeated immunizations with a cocktail containing recombinant human AKT1, AKT2, and AKT3 protein isoforms.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00024-1 is reactive to AKT1, AKT2, AKT3 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
55.686kDa
Background of Akt1
AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00024-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: User optimized
Flow Cytometry: User optimized
IHC: 1:500 - 1:2,000
IF Microscopy: 1:500 - 1:2,000
WB: 1:1,000 - 1:5,000
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of AKT1, AKT2, AKT3 using anti-AKT1, AKT2, AKT3 antibody (A00024-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1, AKT2, AKT3 antigen affinity purified polyclonal antibody (Catalog # A00024-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Protein Target Info & Infographic
Gene/Protein Information For AKT1, AKT2, AKT3 (Source: Uniprot.org, NCBI)
Gene Name
AKT1, AKT2, AKT3
Full Name
RAC-alpha serine/threonine-protein kinase
Weight
55.686kDa
Superfamily
protein kinase superfamily
Alternative Names
AKT, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT2, AKT3 AKT1 AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA AKT serine/threonine kinase 1 RAC-alpha serine/threonine-protein kinase|AKT1m|PKB alpha|RAC-PK-alpha|protein kinase B alpha|proto-oncogene c-Akt|rac protein kinase alpha|serine-threonine protein kinase|v-akt murine thymoma viral oncogene homolog 1|v-akt murine thymoma viral oncogene-like protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AKT1, AKT2, AKT3, check out the AKT1, AKT2, AKT3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AKT1, AKT2, AKT3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AKT AKT1 Antibody (A00024-1)
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Customer Q&As
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15 Customer Q&As for Anti-AKT AKT1 Antibody
Question
Is a blocking peptide available for product anti-AKT antibody (A00024-1)?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
We do provide the blocking peptide for product anti-AKT antibody (A00024-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-27
Question
We want to test anti-AKT antibody A00024-1 on human foreskin for research purposes, then I may be interested in using anti-AKT antibody A00024-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
The products we sell, including anti-AKT antibody A00024-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-26
Question
I was wanting to use your anti-AKT antibody for IHC for human foreskin on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human foreskin identification?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
It shows on the product datasheet, A00024-1 anti-AKT antibody has been tested for ELISA, Flow Cytometry, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human foreskin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-21
Question
We have tried in the past anti-AKT antibody for IF on cervix carcinoma in the past. I am using human, and We want to use the antibody for Flow Cytometry next. My lab would like examining cervix carcinoma as well as left adrenal gland in our next experiment. Could give a recommendation on which antibody would work the best for Flow Cytometry?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
I looked at the website and datasheets of our anti-AKT antibody and it seems that A00024-1 has been tested on human in both IF and Flow Cytometry. Thus A00024-1 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-16
Question
Does anti-AKT antibody A00024-1 work for IHC with foreskin?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
According to the expression profile of foreskin, AKT1 is highly expressed in foreskin. So, it is likely that anti-AKT antibody A00024-1 will work for IHC with foreskin.
Boster Scientific Support
Answered: 2019-10-11
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for foreskin using anti-AKT antibody A00024-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-24
Question
Is this A00024-1 anti-AKT antibody reactive to the isotypes of AKT1?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
The immunogen of A00024-1 anti-AKT antibody is anti-AKT Pan Reactive antibody was produced in rabbits by repeated immunizations with a cocktail containing recombinant human AKT1, AKT2, and AKT3 protein isoforms. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-09
Question
I see that the anti-AKT antibody A00024-1 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-03-19
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-03-19
Question
We are currently using anti-AKT antibody A00024-1 for rat tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-19
Answer
The anti-AKT antibody (A00024-1) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-19
Question
Can you help my question with product A00024-1, anti-AKT antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
N. Kulkarni
Verified customer
Asked: 2017-09-20
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00024-1 anti-AKT antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-09-20
Question
Our team were satisfied with the WB result of your anti-AKT antibody. However we have seen positive staining in foreskin cytoplasm. using this antibody. Is that expected? Could you tell me where is AKT1 supposed to be expressed?
N. Roberts
Verified customer
Asked: 2017-04-20
Answer
From what I have seen in literature, foreskin does express AKT1. Generally AKT1 expresses in cytoplasm. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-04-20
Question
We have seen staining in mouse left adrenal gland. Any tips? Is anti-AKT antibody supposed to stain left adrenal gland positively?
E. Zhang
Verified customer
Asked: 2017-01-06
Answer
Based on literature left adrenal gland does express AKT1. Based on Uniprot.org, AKT1 is expressed in left adrenal gland, adrenal gland, muscle ovary, foreskin, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-01-06
Question
Does A00024-1 anti-AKT antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
N. Wu
Verified customer
Asked: 2016-06-13
Answer
You can see on the product datasheet, A00024-1 anti-AKT antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-06-13
Question
See attached the WB image, lot number and protocol we used for foreskin using anti-AKT antibody A00024-1. Please let me know if you require anything else.
B. Bhatt
Verified customer
Asked: 2015-02-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-02-02
Question
Do you have a BSA free version of anti-AKT antibody A00024-1 available?
D. Bhatt
Verified customer
Asked: 2013-03-05
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-AKT antibody A00024-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-03-05
