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Product Info Summary
| SKU: | PA1923 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-MEKK1/MAP3K1 Antibody Picoband®
SKU/Catalog Number
PA1923
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MEKK1/MAP3K1 Antibody catalog # PA1923. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MEKK1/MAP3K1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1923)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MEKK1, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1923 is reactive to MAP3K1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
164 kDa
Calculated molecular weight
164470 MW
Background of MEKK1
MAP3K1 (Mitogen-activated protein kinase kinase kinase 1), also known as MEKK1, MAPKKK1, MEK KINASE or MAP/ERK KINASE KINASE 1, is an enzyme that in humans is encoded by the MAP3K1 gene. Vinik et al. (1995) identified DNA sequence and size polymorphisms in intronic and 3-prime untranslated regions of the mouse Map3k1 gene and the human MAP3K1 homolog. Using these allele-specific polymorphisms, they mapped the Map3k1 gene in an intersubspecific backcross to mouse chromosome 13. They mapped the human MAP3K1 gene to chromosome 5 by somatic cell hybrid analysis. By assaying transfected COS-1 cells, Xia et al. (1998) showed that human MEKK1 activated JNK1 (MAPK8) robustly and p38-alpha (MAPK14) less efficiently, but it had only a marginal effect on ERK2 (MAPK1). MEKK1 directly and specifically interacted with JNKK1 (MAP2K4) and activated JNKK1 in cells and in vitro. Phosphorylation of JNKK1 by MEKK1 disrupted their interaction. MEKK1 and JNK1 competed for binding to JNKK1. Xia et al. (1998) concluded that JNKK1 is the preferred MEKK1 substrate.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1923 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: MCF-7 Whole Cell
IHC: Human Intestinal Cancer Tissue
Validation Images & Assay Conditions
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Anti-MEKK1 antibody, PA1923, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
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Anti-MEKK1 antibody, PA1923, Western blotting
All lanes: Anti MEKK1 (PA1923) at 0.5ug/ml
WB: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 164KD
Observed bind size: 164KD
Protein Target Info & Infographic
Gene/Protein Information For MAP3K1 (Source: Uniprot.org, NCBI)
Gene Name
MAP3K1
Full Name
Mitogen-activated protein kinase kinase kinase 1
Weight
164470 MW
Superfamily
protein kinase superfamily
Alternative Names
Mitogen-activated protein kinase kinase kinase 1;2.7.11.25;MAPK/ERK kinase kinase 1;MEK kinase 1;MEKK 1;MAP3K1;MAPKKK1, MEKK, MEKK1; MAP3K1 MAPKKK1, MEKK, MEKK 1, MEKK1, SRXY6 mitogen-activated protein kinase kinase kinase 1 mitogen-activated protein kinase kinase kinase 1|MAP/ERK kinase kinase 1|MAPK/ERK kinase kinase 1|MEK kinase 1|mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP3K1, check out the MAP3K1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP3K1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MEKK1/MAP3K1 Antibody Picoband® (PA1923)
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Customer Q&As
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1 Customer Q&As for Anti-MEKK1/MAP3K1 Antibody Picoband®
Question
We are currently using anti-MEKK1/MAP3K1 antibody PA1923 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
The anti-MEKK1/MAP3K1 antibody (PA1923) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-10
